中文摘要：

建立了柱前衍生化HPLC法测定虫草多糖中单糖的组成及摩尔比。采用C18柱，以0．1mol／L磷酸盐缓冲液（pH6．7）．乙腈（83：17）为流动相，检测波长254nm。衍生化试剂为1-苯基-3-甲基-5-吡唑啉酮。所测3批样品中虫草多糖由甘露糖、葡萄糖、半乳糖3种单糖组成，单糖的摩尔比约为2：2：1。平均回收率分别为101．0％、98．6％和99．2％，RSD分别为1．9％、1．6％和1．2％。

英文摘要：

An HPLC method with pre-column derivatization was established for the determination of the monosaccharides in Cordyceps sinensis polysaccharide （CPS） and their molar ratio. A C18 column was used with the mobile phase of 0.1mol/L phosphate buffer （pH6.7）-acetonitrile （83 ： 17） at the detection wavelength of 254nm. 1-Phenyl-3-methyl-5-pyrazolone was used as derivation reagent. The result showed that CPS is composed of mannose, glucose and galactose, and their molar ratio was about 2 ： 2 ： 1 in the three tested samples. The average recoveries were 101.0%, 98.6% and 99.2%, with the RSDs of 1.9%, 1.6%and 1.2%, respectively.