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中文摘要：
研制抗胃泌素释放肽(Gastrin-releasing peptide,GRP)单克隆抗体,并且初步评估其在体外对乳腺癌细胞生长的抑制作用.纯化重组的GRP融合蛋白,免疫BALB/c小鼠,取经免疫的小鼠脾细胞与小鼠骨髓细胞SP2/0进行细胞融合,对杂交瘤细胞进行筛选,阳性孔经3次有限稀释法亚克隆,获得稳定分泌GRP抗体的杂交瘤细胞株,进一步从腹水中用硫酸铵沉淀法纯化单抗,并以间接ELISA、Western免疫印迹以及亚类分型等方法进行抗体鉴定,之后在体外用MTT比色法测定该单抗抑制乳腺癌细胞EMT-6生长的作用.结果表明通过筛选获得了一株阳性杂交瘤细胞,命名为W9,这株杂交瘤细胞分泌的单抗具有效价高,特异性好的特点,其亚型为IgG1,体外的细胞实验也表明这种单克隆抗体能够显著地抑制EMT-6细胞的增殖.
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