中文摘要：

选取了HSP65上对自身免疫性炎症疾病有防治作用的两段功能表位P1（179-190）、P2（31-46）,分别以HSP65和CTB为载体蛋白构建高效表达载体pET28a-HSP65-P1P2P1与pET28a-CTB-P1P2P1,以硫酸铵分级沉淀和包涵体洗涤、经DEAE阴离子交换柱层析分离纯化得到HSP65-P1P2P1与CTB-P1P2P1融合蛋白,并用戊二醛一步偶联法将两者偶联形成HpCp复合物。以小剂量滴鼻粘膜免疫高胆固醇膳食诱发产生动脉粥样硬化的新西兰大白兔,结果显示两组融合蛋白均表现出一定的减斑功效,与PBS对照组相比较,主动脉病斑面积分别减少了34.9%和27.9%,为进一步开发能用于临床的抗AS疫苗提供了良好的设计思路,可经进一步优化设计提高其免疫原性,研发为抗动脉粥样硬化疫苗。

英文摘要：

By using two functional epitopes of HSP65 with preventive effects to autoimmune inflammatory disease,high-level expression vectors of pET28a-HSP65-P1P2P1 and pET28a-CTB-P1P2P1 were built with HSP65 and CTB as a carrier protein respectively.The fusion protein of HSP65-P1P2P1 and CTB-P1P2P1 were acquired by anion exchange column chromatography and then used to immunize the New Zealand white rabbits with atherosclerosis disease induced by high cholesterol diet-fed.The animals were nasally immunized with low-dose proteins for multiple times.The results showed that two groups of fusion proteins possessed the effectiveness of reducing atherosclerotic plaque formation.Compared with the PBS control group the aortic lesion areas of the two groups decreased by 34.9% and 27.9% separately.The study provided a good design idea for the further development of clinical anti-AS vaccine.After further optimization design for increasing their immunogenicity,it could be developed as a vaccine against atherosclerosis.