中文摘要：

目的观察一种新型人甲状旁腺素相关肽（8L-hPTH）对去卵巢大鼠骨质疏松的治疗作用。方法88只3月龄SD健康雌性大鼠其中64只行双侧卵巢摘除术16只做假手术。3个月后各取8只测定腰椎及股骨骨密度。将余下的56只卵巢摘除大鼠随机分为7组每组8只,分别为8L-hPTH高、中、低剂量治疗组（剂量分别为20,10,5μg·kg^-1·d^-1）;hPTH（1-34）高、中、低剂量对照组（剂量分别为20,10,5μg·kg^-1·d^-1）;生理盐水组（0.5mL·d^-1）,各组均为皮下注射。假手术组余下的8只以及剩余8只未做任何手术的大鼠,给药方式及剂量均同生理盐水组。连续给药3个月后,比较各组大鼠股骨、腰椎骨密度并用扫描电镜观察股骨情况。结果双侧卵巢摘除3个月后,大鼠腰椎及股骨骨密度均明显低于假手术组（P〈0.01）,说明造模成功。给药3月后,8L-hPTH各治疗组大鼠与生理盐水组相比较,股骨、腰椎骨密度均显著升高（P〈0.05）,骨小梁宽度有极显著增加（P〈0.001）,效果呈剂量依赖性。结论皮下注射8L-hPTH（5～20μg·kg^-1·d^-1）对去卵巢骨质疏松大鼠有明显的治疗作用。

英文摘要：

OBJECTIVE To evaluate the therapeutic effect of a novel recombinant human parathyroid hormone related-peptide on ovariectomized rats. METHODS Sixty-four of 88 3-month-old female Sprague-Dawley rats were ovariectomized and 16 received sham operation.Three months later,8 rats from each group received bone material density （BMD） test. Then the remaining 56 ovariectomized rats were randomly assigned to 7 groups：control group （NS,0.5 mL normal saline）,8L-hPTH groups （20,10,5 μg·kg^-1）,hPTH（1-34） groups （20,10,5 μg·kg^-1）. Another 8 rats without any operation and sham-operated rats （sham） were treated in the same way as those of group NS. All rats were subcutaneously injected in consecutive days for 3 months. The femoral and lumbar BMD were measured and the femurs were observed by scanning electron microscope after 3-month-therapy. RESULTS Three months later,the femoral and lumbar BMD of ovariectomized rats were significantly decreased,compared with those of sham-operated rats （P〈0.01）. Furthermore,the femoral and lumbar BMD and trabecular widths of 8L-hPTH therapeutic groups were all increased obviously compared with those of group NS （P〈0.05 and P〈0.01）. CONCLUSION 8L-hPTH is effective in stimulating bone formation and improving skeletal microarchitecture. 8L-hPTH is potentially a effective therapeutical agent on osteoporosis.