建立高效液相色谱法分析血样中的3-烟酰胺基-1,2,4-苯并三嗪-1,4-二氧化物(SR—N)。AgilentEclipseC18柱(4.6mm×150mm,5μm),流动相为甲醇-水-冰醋酸=20:80:0.5,流速1.0mL·min^-1,柱温25℃,检测波长277nm。在选定的液相色谱条件下,SR-N血浆标准曲线在0.75~120μg·mL^-1范围内呈良好线性关系(r=0.9994)。检测限和定量限分别为0.25、0.75μg·mL^-1。
To establish a HPLC method for analysis of 3-nicotinamido-1,2,4-benzotriazine-1,4-dioxide(SR-N) in rat plasma, a Agilent Eclipse C18 was used, with mobile phase of methanol-water- acetic acid (20: 80: 0. 5 ) , flow rate 1.0 mL· min^- 1, column temperature 25 ℃, UV detection wavelength 277 nm. The linear ranger of blood sample was 0. 75 - 120 μg ·mL^-1( r = 0. 999 4). the limit of quantification concentration (LOQ) and the limit of quantity concentration (LLOQ) in plasma was 0. 25, 0. 75 μg · mL^-1.