中文摘要：

目的：建立高效液相色谱法测定一种生姜油剂（软胶囊）中6-JF的含量。方法：采用DiamonsilTMC18（4.6mm×250mm,5μm）色谱柱;流动相A为甲醇-异丙醇（50：15）;流动相B为水-冰乙酸（56：4）;流速1.0mL/min;检测波长280nm;进样体积20μL;柱温40℃;线性梯度洗脱。结果 ：检测限浓度为1.24μg/mL,定量限浓度为4.96μg/mL;6-JF在0.044~0.153mg/mL浓度范围内与峰面积呈良好的线性关系（r=0.9995）;平均回收率为100.34%,RSD为1.30%（n=9）。结论：该方法简便,精密度高,结果准确可靠。

英文摘要：

Objective： The method for the determination of 6-gingerol in soft capsules by HPLC is developed.Methods： The sample was separated on a DiamonsilTMC18 column（4.6mm×250mm,5μm）,by a gradient elution using methanol-isopropyl alcohol（50：15,A） and water-glacial acetic acid（56：4,B） as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was 280nm.The volume of sample injection was 20μL and column temperature was 40°C.Results： The limit of detection was 1.24μg/mL and limit of quantitation was 4.96μg/mL.The linear range for 6-gingerol was 0.044~0.153mg/ml（r = 0.9995,n=6）.The average recovery（n = 9） was 100.34%,and RSD was 1.30%.Conclusion： The method is simple,accurate and with good precision.