中文摘要：

目的观察常氧下高低浓度葡萄糖对不同浓度肝癌HepG2细胞缺氧诱导因子1α（HIF-1α）、HIF-2α表达的影响。方法将HepG2细胞分成高中低三个细胞密度组，常氧下分别在高糖和低糖DMEM中培养16h，采用免疫细胞化学法检测各组HepG2细胞中的HIF-1α和HIF-2α。结果常氧下高糖培养，高、中、低密度组HepG2细胞中HIF-1α的光密度值分别为0．270±0．014、0．318±0．015、0．052±0．012，常氧下低糖培养分别为0．300±0．012、0．242±0．015、0．205±0．018。三组间比较，P均〈0．05。常氧下高糖培养，高、中、低密度组HepG2细胞中HIF-2α的光密度值分别为0．076±0．013、0．175±0．011、0．310±0．014，常氧下低糖培养分别为0．120±0．017、0．1874-0．014、0．347±0．015。三组间比较，P均〈0．05。结论常氧下低糖培养能诱导HepG2细胞中的HIF-1α、HIF-2α高表达；HIF-1α在适中的细胞密度下表达最强，HIF-2α的表达随着细胞密度的增加而降低。

英文摘要：

Objective To investigate the influence of glucose concentration on the expressions of HIF-1 α and HIF- 2αin liver cancer HepG2 cells under normoxia in vitro. Methods The HepG2 cells were divided into high, middle, low cell-density groups ,and cultured in DMEM with high and low concentration of glucose. Immuocytoehemistry were used to detect the expressions of HIF-1α and HIF-2α in each group. Results HIF-1α ligh density value in high, middle, low cell-density groups cultured with high glucose and normoxia were 0. 270 ± 0. 014, 0. 318 ± 0. 015, 0. 052 ± 0. 012, and 0.30 ±0. 012, 0.242 ±0.015, 0. 205 ±0.018 in groups cultured with low glucose, P 〈0.05 all. HIF-2αigh density value in high, middle, low cell-density groups cultured with high glucose and normoxia were 0. 076 ± 0. 013, 0. 175 ± 0.011, 0.310 ± 0.014, and 0. 120 ± 0.017, 0. 187 ± 0. 014, 0. 347 ± 0.015 in groups cultured with low glucose, all P 〈 0.05. Conclusions HIF-1α,HIF-2α express highly in HepG2 cells culture with normoxia an low glucose. HIF-1α express most highly in middle cell-density, and HIF-2α expression decrese with cell-density raise.