中文摘要：

目的观察不同浓度的互隔交链孢霉素（altenuene，ALT）对小鼠胚胎成纤维细胞（NIH／3T3）中cAMP水平的影响。方法以NIH／3T3为研究对象，观察以0、2．5、5、10、15和20μmol／L ALT作用30min，并设0．4mmol／L烷化剂甲璜酸甲酯（methyl methanesulfonate，MMS）为阳性参照物，使用超声法破碎细胞，以^125I标记的放射免疫分析（RIA）检测细胞内cAMP水平。结果与对照组相比，5、10、15和20μmol／L ALT能升高细胞内cAMP水平（P〈0．05）；而以0．4mmol／L MMS处理细胞，cAMP水平同样升高。结论ALT对细胞具有明显的影响，可能与经由第二信使cAMP所介导的相关信号通路有关。

英文摘要：

Objective To observe the effects of Ahenuene of different concentrations on cAMP in NIH/3T3 cell line. Methods NIH/3T3 cells were cultured for 30 rain in the medium supplemented with ALT at 0, 2.5, 5, 10, 15, 20 μmol/L and MMS at 0.4 mmol/L for positive control. Then the level of cAMP in NIH/3T3 cell was observed by ^125I-cAMP RIA. Results After NIH/3T3 cells were exposed to different concentrations of ALT for 30 min, the cellular level of cAMP was significantly increased in a concentration-dependent, and in positive control group the level of cAMP increased the same （P 〈 0.05 ）. Conclusion ALT can increase the concentrations on cAMP in NIH/3T3 cells, which related to the single pathway via the second messenger cAMP possibly.