中文摘要：

目的：探讨互隔交链孢酚（AOH）诱导小鼠胚胎成纤维细胞NIH3T3中DNA聚合酶β（DNA pol／3）表达增高的分子信号通路。方法：①15wmol／LAOH作用NIH3T3细胞16h，利用Western blotting方法检测细胞中DNApolβ的表达，并利用磷酸化CREB抗体检测AOH作用后NIH3T3细胞中CREB信号分子是否被激活。②利用PKA-CREB信号通路特异性抑制剂H89预处理NIH3T3细胞1h，再加入AOH作用，分别检测磷酸化CREB和DNA polβ表达的变化。结果：①与溶剂对照组相比，AOH诱导NIH3T3细胞中DNA polβ表达明显增高，而且磷酸化CREB蛋白含量也明显增加，差异均显著。②用PKA特异性抑制剂H89预处理，可部分抑制NIH3T3细胞中CREB蛋白的磷酸化，并且降低了AOH引起的NIH3T3细胞中DNApolβ的表达增加。结论：PKA-CREB信号通路在AOH诱导的NIH3T3细胞中DNApolβ表达起萤要作用。

英文摘要：

AIM： To investigate the signal transduction induced DNA polymerase β expression by alternariol （AOH） in NIH3T3 cells. METHODS： The NIH3T3 cells were treated with 15 μmol/L AOH for 16 h or the cells were pretreated with an inhibitor of PKA （H89） for 1 h, and then exposed to AOH. The changes of DNA polβ expression and phospho - CREB in the ceils with different treatments were determined by Western blotting and immunocytochemistry analysis. RESULTS ： The expression of DNA polymerase βand phospho - CREB in NIH3T3 cells induced by AOH were significantly higher than that in control group （ P 〈 0. 05 ）. However, H89 partly blocked the AOH - induced phospho - CREB and DNA polβ expression. CONCLUSION： Altemariol up - regulates DNA polymerase β expression via the PKA - CREB pathway in NIH3T3 cells.