中文摘要：

以蒙古冰草（Agropyron mongolicum）为研究材料,利用同源序列法克隆得到1个DREB3类转录因子基因,命名为MwDREB3,采用实时荧光定量RT-PCR技术分析MwDREB3基因的表达特征,为蒙古冰草优异抗性基因的挖掘和利用提供理论依据.结果表明：该基因全长1056bp,包含1个完整的开放阅读框,长度为774bp,编码257个氨基酸,该蛋白近5＆#39;端含有1个保守的AP2结构域.系统进化树分析表明MwDREB3编码的蛋白与小麦（Triticum aestivum）的DREB、山羊草（Aegilops columnaris）DRF1的进化关系较近.该基因在干旱、高盐、ABA诱导条件下,表达量上调;在低温条件下,表达量下调.

英文摘要：

To explore and exploit the excellent resistance gene resources of Mongolian wheatgrass（Agropy- ton Mongolicum Keng）, A DREB3 transcription factor gene was cloned by homologous sequences from the Mongolian wheatgrass, named MwDREB3（1056bp）, and the open reading frame was 774bp, encoding 257 amino acids, containing a conserved AP2 domain nearby the 5r end. Phylogenetic analysis revealed that MwDREB3 had a closer relationship with DREBs from wheat （Triticum aestivum） and Aegilops （Ae- gilops columnaris）. Expression patterns of MwDREB3 gene were analyzed using real-time quantitative RT-PCR, the MwDREB3 expression was up under the conditions of drought, high salt and ABA induc- tion, and was down under the low temperatures.