中文摘要：

为分析鳜鱼（Siniperca chuatsi）生肌调节因子基因（mrf4）的特征，阐明其在鳜鱼不同组织以及胚胎发育不同时期的表达规律，用反转录PCR法扩增鳜鱼mrf4的cDNA序列，并将其连入克隆载体，进行测序、分析；用荧光定量PCR检测mrf4基因在不同组织及胚胎发育不同阶段的表达情况。结果：克隆的鳜鱼mrf4 cDNA全长为726bp，编码的蛋白质含241个氨基酸，具有生肌调节因子的典型的碱性螺旋一环一螺旋结构；mrf4在成体鳜鱼白肌、红肌、心脏、脑以及不同发育阶段的胚胎中均有表达，在红肌中的表达量显著高于在其他组织中的表达量（P〈0．05）；在胚胎发育阶段的原肠期、尾芽期、肌肉效应期，mrf4的表达量呈递增趋势，而在胚胎发育的心搏期、血液循环期、仔鱼期，mrf4的表达量呈递减趋势，肌肉效应期mrf4的表达量明显高于胚胎发育的其他阶段的mrf4表达量LP〈0．05）。

英文摘要：

To reveal the characteristics of the mrf4 gene in the mandarin fish and analyze the expression pattern ofmrf4 in different tissues of the adult mandarin fish and in embryos from different developing stages. Reverse transcriptional PCR was used to amplify the mrf4 eDNA sequence of mandarin fish, which was inserted into a cloning vector and sequenced. Quantitative real-time PCR was conducted on samples from different tissues of adult mandarin fish and from embryos of different stages. The results show that the mrf4 gene of mandarin fish is 726 bp in length and encodes 241 amino acids which form a basic helix-loop-helix （bHLH）. Expression ofmrf4 gene was observed in all tested samples including white muscle, red muscle, heart and brain of adult mandarin fish and embryos of different stages. Among the tissue from adult mandarin fish, mrf4 gene expression in red muscle was significantly higher than that in the other tissues （P〈0.05）. For embryos, the expression of the mrf4 increased from the gastrular stage to muscular effect stage, and decreased from the cardiac stage to larval fish with expression in muscular effect stage the highest, which is significantly higher than samples from other stages （P〈0.05）.