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甜瓜白粉病抗性基因遗传分析及定位
  • ISSN号:1004-311X
  • 期刊名称:《生物技术》
  • 时间:0
  • 分类:Q943[生物学—植物学]
  • 作者机构:[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046
  • 相关基金:新疆自治区高技术研究发展计划项目(“基因聚合技术选育哈密瓜抗白粉病新品种”,20111120);国家自然科学基金项目(“甜瓜抗白粉基因发掘与聚合育种”,31060148;“甜瓜抗白粉基因的定位克隆”,31260258)资助
中文摘要:

目的:研究4种甜瓜白粉病抗性品种对白粉病Podosphaera xanthii race 1的遗传规律,对相应抗性基因进行定位。方法:采用,测验法,分析不同分离群体抗、感病植株的分离比,研究其抗性基因的遗传规律。利用BSA(Bulked Segregation Analy-sis)法并结合SSR(Simple Sequence Repeat)分子标记技术,对甜瓜抗性基因进行定位。结果:①抗性亲本PMR45和Km7符合单显性基因的遗传规律(X2=0.133,P=0.72;X2=2.07,P=0.15)。抗性亲本Edist047和红心脆(HXC)均不符合单基因遗传规律,其中Edist047构建的群体符合双基因的遗传规律(r=0.122,P=0.73)。②抗性亲本PMR45和Km7的抗性基因定位于LGⅡ。抗性亲本HXC含有两个抗性基因位点,位于LGV和LGⅫ上。结论:研究了4种白粉病抗性品种所含抗性基因的遗传规律和定位情况,为甜瓜白粉病抗病基因的定位克隆与分子标记辅助育种奠定了基础。

英文摘要:

Objective: Inheritance of 4 sources of Podosphaera xanthii race 1 resistance and location of powdery mildew resistance in melon was studied to explore powdery mildew resistance resources. Method: Inheritance of several resistant sources was observed by analysis of segregation ratios for resistance/susceptibility in different populations. The location of Px race 1 resistance gene was performed by BSA (Bulked Segregation Analysis) and linked SSR (Simple Sequence Repeat) makers technology. Result :①The segregation ratios for resist- ance/ susceptibility observed in the different populations and the results of the progeny test indicated that resistance to Px race 1 in PMR45 and Km7 were governed by one dominant gene (X2 = 0. 133, P = 0. 72 ;X2 = 2. 07, P = 0. 15 ). The resistance in Edisto47 and HXC did not fit to one dominant gene segregation ratio, but Edisto47 would in accord with the closest genetic model of two independent genes (g2 = 0. 122,P =0. 73). ②The powdery mildew resistance in PMR45 and Km7 was located on LG Ⅱ. Two genes were involved in resistance of HXC on LG V and LGⅫ. Conclusion: The genetic analysis and location of four powdery mildew resistance melon cultivars was stud- ied. These results provides a theoretical basis for fine mapping resistance gene and selecting resistance gene in melon breeding.

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期刊信息
  • 《生物技术》
  • 北大核心期刊(2014版)
  • 主管单位:黑龙江省科学院
  • 主办单位:黑龙江省科学院微生物研究所 黑龙江省微生物学会 黑龙江省生物工程学会
  • 主编:张介驰
  • 地址:哈尔滨市道里区兆麟街68号
  • 邮编:150010
  • 邮箱:swjszz@163.com
  • 电话:0451-84615121
  • 国际标准刊号:ISSN:1004-311X
  • 国内统一刊号:ISSN:23-1319/Q
  • 邮发代号:14-225
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  • 被引量:13503