目的:研究4种甜瓜白粉病抗性品种对白粉病Podosphaera xanthii race 1的遗传规律,对相应抗性基因进行定位。方法:采用,测验法,分析不同分离群体抗、感病植株的分离比,研究其抗性基因的遗传规律。利用BSA(Bulked Segregation Analy-sis)法并结合SSR(Simple Sequence Repeat)分子标记技术,对甜瓜抗性基因进行定位。结果:①抗性亲本PMR45和Km7符合单显性基因的遗传规律(X2=0.133,P=0.72;X2=2.07,P=0.15)。抗性亲本Edist047和红心脆(HXC)均不符合单基因遗传规律,其中Edist047构建的群体符合双基因的遗传规律(r=0.122,P=0.73)。②抗性亲本PMR45和Km7的抗性基因定位于LGⅡ。抗性亲本HXC含有两个抗性基因位点,位于LGV和LGⅫ上。结论:研究了4种白粉病抗性品种所含抗性基因的遗传规律和定位情况,为甜瓜白粉病抗病基因的定位克隆与分子标记辅助育种奠定了基础。
Objective: Inheritance of 4 sources of Podosphaera xanthii race 1 resistance and location of powdery mildew resistance in melon was studied to explore powdery mildew resistance resources. Method: Inheritance of several resistant sources was observed by analysis of segregation ratios for resistance/susceptibility in different populations. The location of Px race 1 resistance gene was performed by BSA (Bulked Segregation Analysis) and linked SSR (Simple Sequence Repeat) makers technology. Result :①The segregation ratios for resist- ance/ susceptibility observed in the different populations and the results of the progeny test indicated that resistance to Px race 1 in PMR45 and Km7 were governed by one dominant gene (X2 = 0. 133, P = 0. 72 ;X2 = 2. 07, P = 0. 15 ). The resistance in Edisto47 and HXC did not fit to one dominant gene segregation ratio, but Edisto47 would in accord with the closest genetic model of two independent genes (g2 = 0. 122,P =0. 73). ②The powdery mildew resistance in PMR45 and Km7 was located on LG Ⅱ. Two genes were involved in resistance of HXC on LG V and LGⅫ. Conclusion: The genetic analysis and location of four powdery mildew resistance melon cultivars was stud- ied. These results provides a theoretical basis for fine mapping resistance gene and selecting resistance gene in melon breeding.