中文摘要：

以厚皮甜瓜＂伽师＂为试材,研究了6-苄氨基嘌呤（6-BA）、硝酸银（AgNO3）对诱导不定芽的影响,建立以子叶为外植体的快速高效再生体系。选取5d的＂伽师＂无菌苗子叶,在添加了不同浓度6-BA的MSB培养基中诱导不定芽;并且探讨了在最适6-BA浓度的培养基中添加不同浓度AgNO3对诱导不定芽的影响。结果表明：甜瓜子叶外植体接种在MSB＋1.5mg·L-1 6-BA诱导分化培养基上,诱导率可达75%;添加不同浓度AgNO3诱导不定芽,以MSB＋1.5mg·L-16-BA＋2.0mg·L-1 AgNO3最适,诱导率可达65%,产生单芽较多,缩短了再生周期。丛生芽或单生不定芽转至MSB＋0.1mg·L-1 6-BA＋0.1mg·L-1 GA3的培养基中伸长,不定芽均可伸长且生长状况良好;再将单芽转至MSB＋0.01mg·L-1 NAA培养基进行生根。

英文摘要：

A high efficiency and rapid regeneration system of thick-skinned muskmelon had been set up using cultivars‘Jiashi＇cultured in the media with different hormone of 6-Benzolaminopurine（6-BA）and silver nitrate（AgNO3）.The 5days sterile cotyledon of‘Jiashi＇were cultured in media with different 6-BA concentration.The effect of different AgNO3 concentration added in media with the most appropriate 6-BA concentration was discussed.The results showed that the MSB medium containing 1.5mg·L-1 6-BA was the most suitable for inducing adventitious buds with an average rate of75%.The MSB medium containing 1.5 mg·L-1 6-BA＋2.0 mg·L-1 AgNO3 was the best suitable for inducing adventitious buds with an average rate of 65%,there were most of single adventitious buds,this combination could save time of regeneration system.Adventitious buds were able to elongation on MSB medium with 0.1mg·L-1 6-BA and0.1mg·L-1 GA3,adventitious buds elongated and grew well.Regenerated shoots were rooted on MSB medium with0.01mg·L-1 NAA.