本文采用荧光猝灭法研究了不同pH值和离子强度下胡敏酸与芘的结合常数(Kdoc)的变化,并结合芘的特征参数I1/I3和原子力显微镜图像进行了分析.结果表明,离子强度对Kdoc数值的影响在不同pH值条件下呈现不同的规律.酸性和碱性条件下,Kdoc随着离子强度的增大逐渐减小;而在中性条件下,Kdoc值随离子强度的增大,先增大后减小.
The fluorescence quenching method was used to determine the binding coefficients (Kaoc )between humic acid and pyrene under different pH values and various ion strengths.I1/I3 was employed to analyze the variations of Kdoc combined with images obtained by atomic force microscope. The experiment results showed that ion strengths had significant influence on Kdoc under different pH values. Kaoc decreased with the increasing ion strengths under acidic or base conditions; whereas, Kdoc increased first and then decreased under neutral condition.