中文摘要：

从白果树 biloba 核的 Glycoprotein 可能是过敏的。在这份报纸，过敏蛋白质与西方的弄污被识别，并且 32 kDa glycoprotein 与离子交换层析和胶化层析被净化。与西方的弄污，有有分子的重量的 3 过敏蛋白质 21， 32，和 36 kDa。与 SDS 页分析和蛋白质和糖内容的大小， 32 kDa 的隔离和纯化技术被证实。白果树粗略的蛋白质摘录与硫酸铵被猛抛(浸透坡度：4080%) 。precipitate 被层析与 DEAE 纤维素净化 52，然后，有 Sephadex G-200 的层析和目标 glycoprotein 最后被获得。分析结果证明 glycoprotein 的分子是 32.12 kDa，到糖的蛋白质的比率是 20.56:1。在结论，纯化方法能在 glycoprotein 的准备被使用，并且学习能为 glycoprotein 的进一步的研究提供一个基础。

英文摘要：

Glycoprotein from Ginkgo biloba kernel may be allergic. In this paper, the allergic proteins were identified with Western blotting, and the 32 kDa glycoprotein was purified with ion exchange chromatography and gel chromatography. With Western blotting, there were 3 allergic proteins with molecular weight 21, 32, and 36 kDa. With SDS-PAGE analysis and measurements of the protein and sugar contents, the isolation and purification technology of 32 kDa was confirmed. Ginkgo crude protein extract was precipitated with ammonium sulphate （saturation gradient： 40-80%）. The precipitate was purified by chromatography with DEAE-cellulose 52, then chromatography with Sephadex G-200 and the target glycoprotein was finally obtained. The analysis results showed the molecule of the glycoprotein was 32.12 kDa and the ratio of protein to sugar was 20.56：1. In conclusion, the purification method could be used in preparation of the glycoprotein, and the study could provide a basis for the further research of the glycoprotein.