中文摘要：

通过盆栽实验,研究了不同低温处理条件下香椿叶片可溶性蛋白质含量及组分的变化。结果表明,低温胁迫可以引起香椿叶片蛋白质含量的变化,但不同种源在不同温度下的表现不一致,同一种源在不同胁迫时间下的动态变化也不一致。SDS-PAGE电泳结果显示,抗寒性较强的河南西峡、江苏南京趋种势源,变即化胁规迫律1相天似后,蛋在白-5质℃即低大温量胁表迫达1～,4谱天带过颜程色中变,深可,溶随性后蛋在白第质2、含3量天呈含现量＂逐升渐高下-降降低,谱-再带升变高浅＂,的第变4天化含量又开始增加。两种源分别诱导表达出分子量为27.6kD、22.5kD的特异蛋白。抗寒性较弱的福建霞浦种源可溶性蛋白质含量呈逐渐增加的趋势,但没有诱导出新的蛋白质谱带。各种源SDS-PAGE电泳结果显示的蛋白质谱带颜色的深浅变化与蛋白质含量变化基本一致,抗寒性较强的种源能诱导产生特异蛋白。

英文摘要：

Under low-temperature stress, the changes of protein content and component in leaves ofToona Sinensis were studied by potted experiment. The results indicated that the low-temperature stress could change the content and component in leaves ofToona Sinensis provenances seedlings. Different provenances had different changes under different temperatures. So did the same provenance under different stress periods. SDS-PAGE electrophoresis indicated that low-temperature stress could induce specific proteins’expression. The protein contents of Xixia of Henan and Nanjing of Jiangsu provenances, whose cold resistance were stronger, tended to change as‘high-low-high’pattern during one to four days under -5 ℃. Proteins were induced highly in the first day, and the band became deep. During the second and the third days, it’s the opposite. While in the forth day, it began to express more again. And there were two specific proteins expressed, the molecular weights were 27.6 kD and 22.5 kD for the two provenances respectively. The protein content of Xiapu of Fujian, whose cold resistance was weaker, tended to increase gradually, but had no new proteins appeared. By electrophoresis of SDS-PAGE, the changing of the color of the protein bands changed almost the same with the protein contents. The provenances that had stronger cold resistance could induce specific proteins.