中文摘要：

为提高银杏蛋白的提取率,通过碱法提取银杏种仁中的蛋白质,以蛋白质的提取量为指标,采取混合水平均匀设计研究提取时间、料液比和NaOH溶液质量分数3个因素对银杏蛋白提取效果的影响,通过二次多项式逐步回归分析获得最佳组合。结果表明：最佳工艺条件为：提取时间12h、料液比1：26、NaOH溶液质量分数0.2%,提取量为99.99mg/g,提取率达到了89.14%。超氧阴离子自由基清除率为38.83%,还原能力A700为0.807,表明碱法提取的银杏蛋白具有良好的抗氧化性,可进一步加工利用。

英文摘要：

In order to maximize protein extraction from Ginkgo biloba L.seeds with NaOH solution,a mixed-level uniform design was employed to investigate the effects of time,solid-to-liquid ratio and NaOH concentration on the yield of protein extract.Through quadratic polynomial stepwise regression analysis,the optimum extraction conditions were determined as follows： extraction time 12 h,solid-to-liquid ratio 1：26,NaOH concentration 0.2%.Under such conditions,the yield of protein extract obtained from Ginkgo biloba L.seeds was 99.99 mg/g,and the recovery of protein was 89.14%.The extract obtained showed a superoxide anion free radical scavenging rate of 38.83% and a reducing power A700 of 0.807,thereby having excellent antioxidant effect and being worth further processing and utilizing.