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Effect of Low Temperature Stress on Protein Content of Toona Sinensis Seedlings and Expression of Specific Protein
  • ISSN号:1001-7488
  • 期刊名称:《林业科学》
  • 时间:0
  • 分类:S512.1[农业科学—作物学] Q945.78[生物学—植物学]
  • 作者机构:[1]Department of Life Science,Zhengzhou Normal College,Zhengzhou 450044, [2]College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037
  • 相关基金:Supported by National Natural Science Foundation of China(30872055); Key Scientific and Technological Project of Henan(092102110095)
中文摘要:

[客观] 论文是在低温度的应力下面在 Toona Sinensis 的叶子学习蛋白质内容和部件的变化。[方法] Potted 实验被采用,并且 SDS 页电气泳动也被用来在 T 分析蛋白质的动态变化。Sinensis 离开。[结果] 低温度应力能在 T 的叶子改变内容和部件。Sinensis,而是不同起源在不同温度下面有不同性能,那么在不同压力时期下面做了一样的起源。SDS 页电气泳动结果显示河南省的 Xixia 和有更强壮的冷抵抗的江苏的南京的起源的变化法律是类似的,显示出“ increase-decrease-increase ”的变化趋势。蛋白质极大地为 1 d 在应力以后被表示,乐队的颜色变得更黑暗;内容逐渐地在下列秒和第三天减少了,并且乐队的颜色更轻;内容开始增加了在向前白天。二起源分别地与 27.6 和 22.5 kD 的分子的重量导致了特定的蛋白质。有相对弱的冷抵抗的福建的 Xiapu 的起源的可溶的蛋白质内容逐渐地被增加,但是没有新蛋白质乐队被劝诱。在 SDS 页电气泳动的各种各样的起源的蛋白质乐队颜色的变化与蛋白质内容的变化基本上一致,有更强壮的冷抵抗的起源能导致特定的蛋白质的生产。[结论] 学习提供了理论基础为在分子的机制下面种冷抵抗,它在理论和实践有大意义。

英文摘要:

[Objective] The paper was to study the changes of protein content and component in leaves of Toona Sinensis under low-temperature stress.[Method] Potted experiment was adopted,and SDS-PAGE electrophoresis was also used to analyze the dynamic changes of protein in T.Sinensis leaves.[Result] Low temperature stress could change the content and component in leaves of T.Sinensis,but different provenances had different performance under different temperatures,so did the same provenance under different stress periods.SDS-PAGE electrophoresis results indicated that the change law of provenances of Xixia of Henan and Nanjing of Jiangsu with stronger cold resistance was similar,showing the change trend of "increase-decrease-increase".Protein was greatly expressed after stress for 1 d,the color of band became darker;the content gradually decreased in the following second and third day,and the color of bands was lighter;the content began to increase at the forth day.Two provenances induced the specific proteins with molecular weights of 27.6 and 22.5 kD,respectively.The soluble protein content of provenance of Xiapu of Fujian with relatively weak cold resistance was gradually increased,but no new protein bands were induced.The changes of protein band color of various provenances in SDS-PAGE electrophoresis was basically consistent with the changes of protein content,the provenance with stronger cold resistance could induce the production of specific proteins.[Conclusion] The study provided theoretical basis for under the molecular mechanism of plant cold resistance,which had great significance in theory and practice.

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期刊信息
  • 《林业科学》
  • 北大核心期刊(2011版)
  • 主管单位:中国科协
  • 主办单位:中国林学会
  • 主编:尹伟伦
  • 地址:北京万寿山后中国林学会
  • 邮编:100091
  • 邮箱:lykx@vip.sina.com
  • 电话:010-62889820
  • 国际标准刊号:ISSN:1001-7488
  • 国内统一刊号:ISSN:11-1908/S
  • 邮发代号:82-6
  • 获奖情况:
  • 在三届"国家期刊奖"评选中,两次荣获中国期刊最高奖-"国家期刊奖",一次名列"国家期刊奖提名奖"第一名
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国工程索引,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:42472