中文摘要：

采用荧光光谱法研究了β-环糊精交联聚合物（β-CDP）与盐酸巴马汀（PAL）的相互作用。结果表明，在强碱性条件下β-CDP对PAL能产生显著的荧光增敏作用，据此建立了水溶液中高灵敏测定PAL的荧光光谱法。在最佳条件下，适量PAL溶液，1mL0．001mol／L的β-CDP水溶液，6．0mL10mol／LNaOH溶液，室温下反应，24h内完成测定，测定β-CDP—PAL包合物荧光强度的激发波长364nm，发射波长442nm。测定PAL的线性范围为2～1000ng／mL，检出限为0．65ng／mL。该方法可用于药物制剂及生物体液中PAL含量的测定。

英文摘要：

The binding capacity ofβ-cyclodextrins cross-linked polymer（β-CDP） to palmatine chloride （PAL） has been studied by spectrofluorimetry. The results showed that with PAL in 6.0 mol/L NaOH β-CDP formed stable inclusion complex, meanwhile the fluorescence intensity of PAL increased remarkably. At the optimum reaction conditions, the linear calibration graph were obtained in the concentration range of 2-1 000 ng/mL for the investigated samples and the detection limit was 0.65 ng/mL. The proposed method was applied successfully for the determination of PAL in pharmaceutical dosage form, spiked human urine and spiked plasma.