中文摘要：

目的：建立甲嘎松汤的HPLC特征指纹图谱.方法：选择6-姜辣素和去氢二异丁香酚为对照成分,采用HPLC测定,色谱条件为Platisil ODS色谱柱（4.6 mm× 250 mm,5μm）,流动相乙腈-甲醇-0.3％磷酸水梯度洗脱,检测波长274 nm,柱温25℃,流速1.0 mL·min-1,进样量10 μL.利用“中药色谱指纹图谱相似度评价系统”2004年A版分析10批甲嘎松汤样品的HPLC指纹图谱.结果：共获得15个共有峰,指认了其中2个共有峰分别为6-姜辣素和去氢二异丁香酚.10批样品的指纹图谱相似度均＞0.9,表明相关性较好,所含化学成分基本一致.结论：该法操作简便、灵敏度高、准确可靠,为甲嘎松汤的质量评价提供科学依据.

英文摘要：

Objective： To establish HPLC characteristic fingerprint of Jiagasong decoction. Method： Taking 6-gingerol and dehydrodiisoeugenol as control components, which were derermined by HPLC, chromatographic conditions were as follows： Platisil ODS column （4.6 mm x250 mm, 5 μm） , mobile phase of acetonitrile-methanol-0.3% phosphoric acid solution for gradient elution, detection wavelength 274 nm, column temperature 25 ℃, flow rate 1.0 mL min-1, injection volume 10 μL. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine （2004A） was used in analysis 10 batches of Jiagasong decoction. Result： HPLC characteristic fingerprint of Jiagasong decoction was established, 15 common peaks were received, two of them were identified as 6-gingerol and dehydrodiisoeugenol. Similarity of fingerprint among 10 batches of samples was more than 0.9, indicating a good correlation and basically the same chemical compositions. Conclusion： This assay method was simple, sensitive and accurate, this paper could provide scientific basis for quality evaluation of Jiagasong decoction.