中文摘要：

目的：观察皮质酮（CORT）对培养的脊髓背角神经元Ca2＋浓度（[Ca2＋]i）的调节作用及机制。方法：培养新生sD大鼠脊髓背角神经元，激光共聚焦显微镜检测神经元[Ca2＋]i的变化。结果：CORT可快速升高培养的脊髓背角神经元[Ca2＋]i，且呈现剂量依赖性（P〈0．05）；CORT诱导的神经元[Ca2＋]i升高是以外钙内流为主（P〈0．01）；百日咳毒素（G蛋白活化阻断剂）可阻断CORT所致的脊髓背角神经元[Ca2＋]i升高（P〈0．01），而糖皮质激素受体拮抗剂RU38486对CORT的效应无抑制作用。结论：CORT通过非基因组途径快速增高培养的脊髓背角神经元[Ca2＋]i。

英文摘要：

Objective： To explore the modulatory effects of corticosterone （CORT） on the intracellular calcium concen- tration （ [Ca2＋ ]i） in cultured rat dorsal spinal neurons and the relative mechanism. Methods：Neurons from neonatal SD rat spinal cord were cultured. Confocal laser scanning microscopy were employed to detect changes of [ Ca2＋ ] i in cultured neurons. Results：CORT （0.1-10 μmo]/L） caused a rapid increase in [ Ca2＋ ]i with a dose-dependent manner in cul- tured dorsal spinal neurons. The action of CORT on neuronal [ Ca2＋] i was blocked by pertussis toxin （ a blocker of G protein activation, 100 ng/ml） , but was unaffected by RU38486 （glueocorticoid receptor antagonist, 10 μmol/L）. Con- clusion ： These observations suggest that CORT can rapidly increase [ Ca2＋ ] i in cultured rat dorsal spinal neurons through a nongenomic mechanism.