从多年施用莠去津的栽参土壤中分离出6株可降解莠去津的菌株,其中3株放线菌、2株细菌、1株真菌;对其在田间土壤中的定殖情况和降解效果进行了研究。结果表明,土壤经过消毒处理,有利于放线菌在土壤中定殖。高接种量(12S)与低接种量(6S)处理间差异呈极显著性(P〈0.01);同等接种量条件下,混合菌剂处理(6S5F、5M6S)土壤中放线菌数明显高于单株菌(6S)处理。在田间生产条件下,土著微生物代谢莠去津的生物半衰期分别为76d(栽参)和55d(休闲),土壤中莠去津消失95%所需要的时间由404d提前到132d,消失速率提高了3倍。
Using solid medium, fifty-six strains degrading atrazine were isolated from cultivated Ginseng soil where atrazine was employed for years. Three actinomycete strains, two bacteria strains, and one fungi strain showed higher degradation rate in soil. The optimum temperature and pH value for the six strains were 20~30 ℃ and 6.0~8.0, respectively. In the experiment 6S, 12S, 5M6S, and 6S5F were inoculated into soil as testing microbe strains. Colonization and degradation effect of degrading-bacteria in soil was carried out using split-plot design including four main plots and five split plots. The results showed that sterlized soil was propitious to the breed of actinomycetes. The difference between high( 12S)and low(6S)inoculation levels was significant (P〈0.01) in number and activities of microorganism using SPSS software. The number of actinomycetesin the soil treated by mixed strains (6S15F and 5M6S) was significantly bigger than that in the soil treated by single strain (6S)(P〈0.01) with the same inoculation levels. Bacteria mixtures were fed into the optimized soil condition and periodically monitored the residue of atrazine. The biodegradation of atrazine followed the first order reaction kinetic equation and the differences of correlation coefficients were significant. In field, half lives of biodegradation of atrazine by isolated microorganisms were 76 days (in cultivated soil) and 55 days (in fallow soil), respectively. The time needed to degrade 95% of atrazine varied from 404 days to 132 days with increasing of the 3 factors.