中文摘要：

目的 ：分析CD4＋CD25High、CD4＋CD25＋CD127Low/–、CD4＋CD25＋Fox P3＋3种标记的Tregs细胞比例的相关性,探讨不同荧光素组合标记Tregs细胞的鉴定效果。方法：随机抽取健康体检者60例,平均分成2组（Ⅰ组、Ⅱ组）,Ⅰ组用CD25-APC/CD127-PE/CD4-FITC三色抗体标记,Ⅱ组用CD25-PE/CD127-Alexa Fluor禄R647/CD4-Per CP-Cy5.5标记;另选Ⅰ组10例,用CD25-FITC/Fox P3-PE/CD4-Per CP-Cy5.5/CD127-Alexa Fluor禄R647四色抗体标记;流式细胞仪检测,Cell Quest软件分析,比较Tregs细胞结果差异,分析Fox P3＋与CD127Low/–的相关性。结果：CD25＋CD127Low/–Tregs细胞Ⅱ组（7.89±1.37）%明显高于Ⅰ组（6.37±1.83）%（P=0.001）,2组中CD4＋CD25High和CD4＋CD25＋CD127Low/–的相关系数（r）分别是0.944、0.916;（94.08±1.93）%的CD25＋Fox P3＋细胞低表达CD127,CD25＋Fox P3＋和全血检测CD25＋CD127Low/–的Tregs细胞有较高的相关性（r=0.846）,结果差异不明显（P=0.774）。结论：CD25＋CD127Low/–是Tregs细胞特异性标志,适合临床应用。全血检测Tregs细胞,用CD25-APC、CD127-PE、CD4-FITC荧光素组合检测结果与Fox P3相关度高。

英文摘要：

Objective：To analyze the association of three kinds of Tregs labeled by CD4＋CD25High, CD4＋CD25＋CD127Low/-, CD4＋ CD25＋FoxP3＋ and study the effect of different fluorescence labeling methods on Tregs identification. Methods： Sixty samples of the peripheral blood from 60 healthy people were randomly divided into 2 groups （group I and group Ⅱ ）. Group I was labeled by CD25- APC/CD127-PE/CD4-FITC, group Ⅱ was labeled by CD25-PE/CD127-Alexa Fluor47/CD4-PerCP-CyS.5. Another 10 samples from group I were labeled by CD25-FITC/FoxP3-PE/CD4-PerCP-CyS.5/CD127-Alexa Fluor647. All labeled samples were detected using flow cytometry, and the data were analyzed using CellQuest software to count the numbers of Tregs labeled with different methods, and then the relationships between them were analyzed. Results： CD25＋CD127Low/- Tregs cells in group Ⅱ（7.89 ± 1.37）% was significantly higher than that in group I （6.37 ± 1.83）%（P = 0.001）. The correlation coefficient （r） of CD4＋CD25High and CD4＋CD25＋CD127Low/- in the two group were 0.944 and 0.916, respectively; （94.08 ± 1.93）% of CD25＋FoxP3＋ cells were also labeled with low CD127 expression, there was a high correlation （r=0.846） and no significant difference （P=O.774）between them. Conclusion： CD25＋CD127Low/- can specifically label Tregs, and is suitable for clinical application. Between the labellings with FoxP3 and CD25-APC/CD127-PE/CD4-FITC fluorescein combination, there is a high correlation to identify Tregs in the peripheral blood.