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中文摘要：
利用切刻内切酶的酶切作用实现信号放大,结合量子点高效的电化学发光性能,构建了一种新型电化学发光DNA生物传感器。将捕获探针DNA（c-DNA）通过自组装的方式固定到金电极表面,后与目标DNA（t-DNA）互补杂交形成双链DNA,利用切刻内切酶Nt.Bst NBI特异性识别双链上的酶切位点（5＇-GAGTC-3＇）,然后在c-DNA相应的切割位点（识别序列3＇端后的4个碱基处）对其进行剪切,释放出目标链,参与下一轮的杂交及酶切,通过目标物的循环利用,实现信号放大。利用N-羟基琥珀酰亚胺（NHS）和1-乙基-3-3-二甲基氨丙基碳化二亚胺（EDC）活化羧基化Cd Te量子点表面的羧基,与电极表面残留的c-DNA末端的氨基共价交联,通过测定捕获的量子点的电化学发光信号对目标DNA进行检测。优化后的检测条件为：c-DNA浓度1μmol/L,杂交时间60 min,Nt.Bst NBI浓度0.5 U/μL,酶切反应时间4 h。在优化条件下,目标DNA浓度在2.0×10~（-13）~2.0×10~（-11）mol/L范围内,其对数与电化学发光强度呈线性关系,检出限为7.3×10~（-14） mol/L。人体血样加标回收率为96.4%~108.0%。
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