中文摘要：

以绿麦隆为模板分子,在镍电极表面聚合制得绿麦隆分子印迹膜。洗脱后的分子印迹膜重新吸附不同浓度绿麦隆分子,在印迹膜上形成不同量的印迹孔穴,H2O2通过印迹孔穴在镍电极表面产生电催化氧化,从而形成门控制电催化效应,以此对绿麦隆进行定量分析。研究表明,镍电极对H2O2有较好的催化效果,分子印迹聚合膜中模板分子、功能单体和交联剂的摩尔配比为1∶12∶30,重吸附时间为10 min,测试缓冲液pH=7.2。H2O2的催化电流值与绿麦隆浓度在1.0×10-4～4.0×10-7 mol/L范围内呈良好的线性关系;检出限为2.9×10-8 mol/L。将传感器应用于农田水样的检测,其回收率在97%～103%之间。

英文摘要：

Molecularly imprinted polymer （MIP） was synthesized on the surface of Ni electrode with the template molecules of chlortoluron. After elution, some of the imprinted cavities in MIP can re-bind with chlortoluron molecules. Hydrogen peroxide, which acts as the probe, can enter into the left cavities and are reduced on the Ni electrode surface to form ＂gate controlled electro-reduction＂. The MIP sensor can be used to detect chlortoluron with high sensitivity due to the highly electrochemically catalytic reduction of H2O2 on the Ni electrode. The mole ratio of template molecules, functional monomer and cross- linking agent in molecular imprinted polymer film was found to be 1：12： 30, re-adsorption time was 10 rain and the optimum pH value was 7.2. Chlortoluron could be analyzed in the range of 1.0× 10-4 --4.0× 10-7 mol/L with a detection limit of 2. 9 × 10-8 mol/L. In addition, the feasibility of the practical applications of the MIP sensor has been demonstrated in the analysis of water samples and showed good recovery, ranging from 97% to 103%.