中文摘要：

目的：探讨表皮生长因子受体（EGFR）抑制剂对外源锌离子致大鼠呼吸道急性炎症的影响。方法：将100μmol/L硫酸锌溶液经气管灌注大鼠肺部复制动物炎症模型,于灌注后4h、8h和24h处死大鼠,以PBS为对照,分别测定支气管肺泡灌洗液（BALF）中白细胞计数、总蛋白、白细胞介素8（IL-8）以及乳酸脱氢酶（LDH）的含量,以选择炎症最明显的时间点。进一步用二甲基亚砜（DMSO）和EGFR抑制剂PD153035预灌注大鼠,观察其对硫酸锌溶液所致呼吸道炎症的影响。结果：不同灌注液作用后大鼠白细胞计数（F=12.149,P=0.002）和IL-8（F=48.680,P〈0.001）等炎症反应指标不同,不同时间点白细胞计数（F=29.569,P〈0.001）和IL-8（F=59.778,P〈0.001）等炎症反应指标也不同,硫酸锌灌注后8h为炎症反应最强的时间。不同预灌注液作用后大鼠白细胞计数（F=37.872,P〈0.001）和IL-8（F=24.347,P〈0.001）等炎症反应指标不同,PD153035能抑制炎症反应的强度。结论：EGFR抑制剂能有效抑制外源锌离子所致大鼠呼吸道急性炎症。

英文摘要：

Aim：To determine the effects of EGFR inhibitor on zinc-induced acute airway inflammation in rats.Methods：Use acute airway inflammation animal model to ascertain the time points with the severest airway inflammation.100 μmol/L zinc sulfate was tracheally instilled into rat lungs with PBS as control.The rats were sacrificed 4,8,and 24 h after the instillation and lung lavage was conducted thereafter.The number of white blood cells and the levels of proteins,LDH,and IL-8 in lung lavage fluids were determined,respectively.Moreover,rats were pre-instilled with the EGFR inhibitor PD153035 prior to zinc sulfate instillation and the alterations in the severity of airway inflammation observed.Results：Different perfusates cause white blood cell number （F was 12.149,P was 0.002） and IL-8 （F was 48.680,P0.001） and other indicators of rats inflammation different,white blood cell number （F was 29.569,P0.001 ） and IL-8 （F was 59.778,P0.001） and other indicators of inflammation at different time points were different,inflammation was the strongest after zinc sulfate perfusion 8 h.Different pre-perfusates cause white blood cell number （F was 37.872,P0.001） and IL-8 （F was 24.347,P0.001） and other indicators of rats inflammation different,PD153035 can suppress the inflammation.Conclusion：The EGFR inhibitor can suppress zinc sulfate-induced airway inflammation.