中文摘要：

目的探讨硫酸锌对人呼吸道上皮细胞内转录因子AP-1及NF-κB活性的影响。方法应用蛋白质免疫印迹及基因转染实验,以人呼吸道上皮细胞株BEAS-2B作为体外模型,分别检测硫酸锌对AP-1的磷酸化作用和对AP-1转录活性的影响以及硫酸锌对IκBα蛋白的降解作用和对NF-κB转录活性的影响。结果硫酸锌刺激可诱导AP-1亚单位c-Jun的磷酸化及AP-1转录活性的升高,且均呈现时间-效应关系,其中8hAP-1转录活性升高为1h的16倍。在同一剂量下,硫酸锌虽未能诱导IκBa蛋白的降解,但仍能提高NFκB的转录活性,提示硫酸锌可能通过非IκBa途径诱导NF-κB的活化。结论硫酸锌可激活呼吸道上皮细胞内转录因子AP-1及NF-κB。

英文摘要：

Objective To explore the effects of zinc sulfate on activities of transcription factors AP-1 and NF-κB. Methods A human airway epithelial cell line （BEAS-2B） was used as the in vitro cell line. Immunoblotting and plasmid transfeetion were utilized to determine phosphorylation of c-Jun and AP-1 reporter activity, respectively. The same assays were also used to measure bob protein levels and NF-κB reporter activity. Results Exposure to zinc sulfate induced time-dependent increase in c-jun phosphorylation and AP- 1 reporter activity. The AP-1 transcriptional activity at 8 h exposure increased to16 fold of that at 1 h exposure. Under the same treatment conditions, zinc sulfate stimulation failed in inducing degradations of boB protein, but still increased NF-κB transcriptional activity, implying that zinc sulfate could activate NF-κB through an IκB-independeut pathway. Conclusion Zinc sulfate could activate transcription factors AP-1 and NF-κB in human airway epithelial cells.