中文摘要：

目的探讨白细胞介素（IL）-17F及IL-17受体c（IL-17RC）对大鼠肺微血管内皮细胞（PMVEC）小窝蛋白1（Cav-1）表达的影响。方法将体外培养的大鼠PMVEC分为时效实验组以及IL-17RC信号通路干预实验组；根据时效实验组结果：即IL-17F诱导大鼠PMVEC的Cav．1及其磷酸化表达峰值的时间，制定IL-17RC信号通路干预实验组IL．17F与大鼠PMVEC孵育时间：（1）时效实验组：100n/ml的IL-17F刺激0、0．5、1．5、3．0、6．0、12．0、24．0h后Western印迹法检测Cav．1表达以及0、10、30、60、90、120rain后检测Cav-1及其磷酸化（p-Cav-1）的表达；（2）IL-17RC信号通路干预实验组：sitlNA病毒与大鼠PMVEC预孵育3d后分成2组，第1组给予100ng／ml的IL-17F刺激60min后检测p-Car-1表达，第2组给予100rig／ml的IL-17F刺激24．0h后检测Cav．1表达，各组分别设对照组、无意义-siRNA组、IL-17RC-siRNA组、IL-17F组以及无意义-siRNA±IL-17F组作为参照。结果IL-17F时间依赖性增强大鼠PMVEC表达Cav-1，0、0．5、1．5、3．0、6．0、12．0、24．0h相对表达量分别为（1．139±0．134）、（1．276±0．166）、（1．604±0．080）、（2．115±0．231）、（2．763±0．226）、（3．309±0．493）、（3．963±0．169），1．5h时开始显著高于0h，24．0h时达峰值且显著高于0、0．5、1．5、3．0、6．0、12．0h（均P〈0．05）；IL-17F时间依赖性增强p-Cav-1表达，0、10、30、60、90、120min相对表达量分别为（0．540±0．085）、（0．880±0．084）、（1．437±0．297）、（1．491±0．212）、（1．017±0．210）、（0．882±0．074），10rain时开始显著高于0rain，60rain时达峰值且显著高于0、10、30、90、120rain（均P〈0．05），然后逐渐下降，120rain时仍高于0rain。IL-17RC-siRNA可显著抑制IL-17F诱导的Cav-1和P-Car-1的表达（2．126±0．318比3．897±0．424和1．014±0．136比1．431±0．298，均P〈0．05）。结论IL-17

英文摘要：

Objective To explore the expression of caveolin-1 （Cav-1） induced by interleukin- 17F/IL-17 receptor C （IL-17F/IL-17RC） in rat pulmonary microvascular endothelial cells （PMVECs）. Methods Cultured PMVECs were divided into two groups of time-dependent experiment and IL-17RC signal pathway intervention according to the results of time-dependent experimental group ~ namely peak time of IL-17F-induced Car-1 and its phosphorylation （p-Cav-1） expression of PMVEC for formulating the incubation time between IL-17F and PMVEC in IL-17RC signal pathway intervention group. Time-dependent experiment group： Western blot was used to detect the expression of Car-1 after IL-17F stimulation for 0, 0. 5, 1.5, 3.0, 6. 0, 12. O, 24. 0 h. Cav-1 and its phosphorylation expression after IL-17F challenge for 0, 10, 30, 60, 90, 120 min were evaluated by Western blot. IL-17RC signal pathway intervention group： PMVECs were divided into two groups after a 3-day pre-treatment of siRNA. The first group received a 60- min stimulation of 100 ng/ml IL-17F before detecting the expression of p-Cav-1 while the second group was subject to a 24-hour stimulation of 100 ng/ml IL-17F before detection. In addition, control, meaningless-siRNA, IL-17RC-siRNA, IL-17F and meaningless-siRNA ± IL-17F groups were set as references for two groups respectively. Results IL-17F up-regulated the expression of Cav-1 in a time-dependent manner. At 0, 0.5, 1.5, 3.0, 6.0, 12.0, 24.0 h, the relative expression levels of Cav-1 were （1. 139 ±0. 134）, （1.276±0.166）, （1.604 ±0.080）, （2.115 ±0.231）, （2.763 _±0.226）, （3.309 ±0.493） and （3. 963 ± 0. 169）. At 1.5 h, it was significantly higher than 0 h, peaked at 24.0 h and remained significantly higher than 0, O. 5, 1.5, 3.0, 6. 0, 12. 0 h （ all P 〈 0.05 ）. And IL-17F increased the expression of p-Cav-1 in a time-dependent manner. At O, 10, 30, 60, 90, 120 min, the relative expression levels of p-Car-1 were （0.540 ±0.085）, （0.880 ±0.084）, （1.437 ±0.297）,