中文摘要：

为了获得长片段（GAA）n·（CTT）n重复廖列进而对其结构特性和致病机理研究，通过化学合成重复序列及两侧接头。两侧接头有Eco R I，Barn H I和Ple I酶切位点。Eco R I，Barn H I位点用于将重复序列插入pUC19制成的载体，Ple I酶切位点可方便地将重复序列从重组质粒中取出．为核苷酸重复序列的动力学结构和探讨与遗传病相关的重复序列结构特性及该类遗传疾病的分子机理奠定了基础．

英文摘要：

To clone （GAA）n·（CTT）n repeats for the further investigation on structural feature and mechanism with repeats sequence causing diseases.There exist EcoR I,BamH I and Ple I sites in the chemically synthesized repeat sequences and adaptors. The site of EcoR I and Bam H I were used to insert repeats sequences into vector made from pUC19,while Ple I sites can be used to take out the repeat sequences from recombinant plasmid easily. This work laid the foundation for the study of dynamic structure of nucleotide repeat sequences, and for the further investigation on structural properties of repeat sequences and molecular mechanism of genetic diseases.