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Fluorescence spectroscopy characteristics of humic acid by inoculating white-rot fungus during different phases of agricultural waste composting

ISSN号：1002-7432
期刊名称：《热固性树脂》
时间：0
分类：S153.622[农业科学—土壤学;农业科学—农业基础科学] TQ424.25[化学工程]
作者机构：[1]College of Environmental Science and Engineering, Hunan University, Changsha 410082, China
相关基金：Project（2005CB724203） supported by the Major State Basic Research and Development Program of China; Project（IRT0719） supported by the Program for Changjiang Scholars and Innovative Research Team in University of China; Projects（50608029, 50808073） supported by the National Natural Science Foundation of China; Project（2007185） supported by the Environmental Protection Technology Research Program of Hunan Province, China

作者：黄红丽[1], 曾光明[1], 蒋荣清[1], 袁兴中[1], 喻曼[1], 黄丹莲[1], 张嘉超[1], 冯冲凌[1]

关键词：荧光光谱特性, 农业废物, 白腐菌, 腐殖酸, 堆肥, 接种, 二次发酵, 腐殖化程度, composting, inoculation, Phanerochaete chrysosporium, humic acid, fluorescence spectroscopy

中文摘要：

白腐烂真菌， Phanerochaete chrysosporium (P。chrysosporium ) ，在腐殖的酸的荧光光谱学特征上在农业浪费 composting，和它的效果的不同阶段期间被接种(哈) 被学习。结果证明排放系列在 400 nm 和一个宽广肩膀有一座锋利的山峰，最大值在 460 nm 集中了。刺激系列在 470 nm 有二山峰和展览红移动(到更长的波长的移动) 。同步扫描系列介绍很多座山峰和肩膀，并且在更短的波长的山峰逐渐地消失并且形成一个肩膀。在 composting 的最后的阶段，荧光系列有类似的形状，但是荧光紧张减少。P。chrysosporium 增加 aromatization 的度和聚合哈当它在第二发酵期间被接种时，分阶段执行，当它不在 humification 度上生产一个明显的变化时哈当它在第一发酵期间被接种时，分阶段执行。与从土壤的 HA 的荧光光谱学特征相比，从堆肥的 HA 的结构更简单，这项活动更高。

英文摘要：

The white-rot fungus, Phanerochaete chrysosporium （P. chrysosporium）, was inoculated during different phases of agricultural waste composting, and its effect on the fluorescence spectroscopy characteristics of humic acid （HA） was studied. The results show that the emission spectra have a sharp peak at 400 nm and a broad shoulder with the maximum centered at 460 nm. The excitation spectra have two peaks and exhibit red shift （shift to longer wavelengths） at 470 nm. The synchronous scan spectra present a number of peaks and shoulders, and the peaks at shorter wavelengths disappear gradually and form a shoulder. At the final stage of composting, the fluorescence spectra have similar shapes, but the fluorescence intensities decrease. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase. Compared with the fluorescence spectroscopy characteristics of HA from soil, the structure of HA from compost is simpler and the activity is higher.

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