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Laccase biosensor using magnetic multiwalled carbon nanotubes and chitosan/silica hybrid membrane modified magnetic carbon paste electrode

ISSN号：1673-9108
期刊名称：《环境工程学报》
时间：0
分类：O657.15[理学—分析化学;理学—化学] O657.1[理学—分析化学;理学—化学]
作者机构：[1]College of Environmental Science and Engineering, Hunan University, Changsha 410082, China, [2]Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education, Hunan University, Changsha 410082, China
相关基金：Project（IRT0719） supported by the Program for Changjiang Scholars and Innovative Research Team in University, China; Projects （50978088, 51039001） supported by the National Natural Science Foundation of China; Project（2009FJ1010） supported by the Hunan Key Scientific Research Program, China; Project（10JJ7005） supported by the Natural Science Foundation of Hunan Province, China; Projects（CX2009B080, CX2010B 157） supported by the Hunan Provincial Innovation Foundation For Postgraduate; Project supported by the Fundamental Research Funds for the Central Universities, Hunan University, China

作者：庞娅[1,2], 曾光明[1,2], 汤琳[1,2], 章毅[1,2], 李贞[1,2], 陈丽娟[1,2]

关键词：壳聚糖/二氧化硅杂化膜, 酶生物传感器, 多壁碳纳米管, 碳糊电极, 磁性, 化学共沉淀方法, 振动样品磁强计, 扫描电子显微镜, magnetic multiwalled carbon nanotubes, paramagnetism, chitosan/silica sol, laccase biosensor, catechol

中文摘要：

为儿茶酚的决心构造 laccase 生物传感器的简单、快速的策略被调查。拥有电子转移的优秀能力的磁性的 multiwalled 碳 nanotubes (MMCNT ) 被化学一起沉淀方法准备。扫描电子显微镜(SEM ) 和颤动的样品磁强计(VSM ) 被用来分别地识别它的 surfacetopography 和磁化。Laccase 在 MMCNT 上被使不能调动由 chitosan/silica (CS ) 的帮助的修改的磁性的碳浆糊电极混合的膜。用当前时间的察觉方法，生物传感器从 107 ～ 0.165 椠 ? 慀牲慥獲眠在范围显示出与儿茶酚的集中有关的线性回答吗？

英文摘要：

A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled carbon nanotubes （MMCNT） which possess excellent capability of electron transfer were prepared by chemical coprecipitation method. Scanning electron microscope （SEM） and vibrating sample magnetometer （VSM） were used to identify its surfacetopography and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid of chitosan/silica （CS） hybrid membrane. Using current-time detection method, the biosensor shows a linear response related to the concentration of catechol in the range from 10-7 to 0.165×10-3 mol/L. The corresponding detection limit is 3.34× 10-8 mol/L based on signal-to-noise ratios （S/N） ≥3 under the optimized conditions. In addition, its response current retains 90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop other enzyme-based biosensors.

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