采用接种培养方法研究了不同木质素降解菌对木质素降解率、腐殖质总量、各组分含量及胡敏酸E4/E6的影响。结果表明,接种黄孢原毛平革菌和栗褐链霉菌对稻草的降解均有所提高,56天培养后,二者的木质素降解率分别达到40.86%和31.04%,而对照组(只含有土著微生物)只有10.56%。接种黄孢原毛平革菌和栗褐链霉菌显著提高了腐殖质产量,这两种不同木质素降解菌产生腐殖质的最大值分别是对照组的2.10和2.13倍,但二者降解木质素形成腐殖质的途径有所不同。培养结束后,各条件下土壤中胡敏酸E4/E6均有所增加.表明胡敏酸的芳构化程唐有所减弱。
To study the effects of two different ligninolytic organisms, Phanerochaete chrysosporium and Streptomyces badius, on humus formation, the degradation rate of lignin, the amount of humic extrac (HE), humic acid(HA), fulvic acid (FA) and the E4/E6 of HA were analyzed during an incubation process. The results show that the two lignolytic organisms can stimulate the degradation of lignin. The lignin degradation rate is only 10.56% in the control while those in the treatments P. chrysosporium and S. badius are 40.86% and 31.04%, respectively. It is also found that inoculating ligninolytic organisms significantly enhance the coment of humus and the pathways of humus formation from lignin by the two inoculants are different. The maximum amounts of humus formed by P. chrysosporium and S. badius are 2. l0 and 2.13 times that of the control, respectively. After the incubation, the E4/E6 of HA increase, which indicates a reduction of the aromatization of HA.