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中文摘要：
以B淋巴细胞表面CD20抗原靶向的单克隆抗体Rituximab为载体，通过共价键偶联荧光基团菁染料Cy7，获得了新型荧光分子探针Cy7-Rituximab．利用全光谱紫外-可见分光光度仪、SDS-聚丙烯酰胺凝胶电泳和基质辅助激光解析电离飞行时间质谱等对该探针结构进行表征，并通过激光共聚焦显微镜观察了其在弥漫大B细胞淋巴瘤（DLBCL）细胞中的摄取情况．选用BALB／C裸鼠为模型，尾静脉注射Cy7-Rituximab，通过活体荧光成像系统观察了其在小鼠体内的分布情况．研究结果表明，修饰后的Cy7-Rituximab保持了原有抗体的免疫活性．活体荧光成像结果表明，在CD20高表达的脾脏部位监测到该分子探针的特异性浓集．
英文摘要：
A new fluorescence molecular imaging probe Cy7-Rituximab was synthesized through the connec- tion of fluorescence Cy7 dye and protein Rituximab. The probe was fully characterized by fluorescence spectro- photometer, SDS-PAGE and confocal microscopy. The uptake of Cy7-Rituximab probe in DLBCL cells was ob- served by laser scanning confocal microscope. CyT-Rituximab probe of 0. 4 mL（0. 6 mg/mL） was injected to BALB/C nude mice through tail vein. The biodistribution of the novel probe CyT-Rituximab was eventually observed, especially its specific concentration in spleen.
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