中文摘要：

目的：比较正常与炎症来源牙周膜干细胞（H-PDLSCs与P-PDLSCs）中乙酰基转移酶MORF表达水平的差异及其对PDLSCs分化的调控。方法：有限稀释法培养H-PDLSCs与P-PDLSCs,LPS、TNF-α、IL-β及三者混合物体外模拟炎症微环境诱导H-PDLSCs（IP-PDLSCs）;基因与蛋白检测方法对比2种来源PDLSCs中MORF的表达水平;蛋白检测方法检测IPPDLSCs中MORF的表达水平;基因、蛋白检测与茜素红染色方法对比H-PDLSCs及下调MORF基因后的PDLSCs成骨基因表达的差异。结果：与H-PDLSCs相比,P-PDLSCs中MORF的表达显著下降（P〈0.05）;IP-PDLSCs中MORF的表达下降;MORF基因被下调后,PDLSCs成骨能力下降（P〈0.05）。结论：牙周炎及炎症微环境会导致PDLSCs中MORF表达的下降,从而使其成骨分化受到抑制。

英文摘要：

Objective： To compare acetyltransferase MORF level in periodontal ligament stem cells（PDLSCs） derived from healthy individuals （H-PDLSCs） with those derived from the individuals with periodontitis （P-PDLSCs）. And to determine the effect of MORF on the osteogenic differentiation potential of PDLSCs. Methods： Human H-PDLSCs and P-PDLSCs were cultured and cloned with limited dilution method. H-PDLSCs were stimulated by LPS, TNF-or, IL-13 and the mix of the 3 inflammatory factors to imitate inflammatory environment（IP-PDLSCs）. Quantitative RT-PCR and Western Blot were applied to examine different expression of MORF in H-PDLSCs and P-PDLSCs. Western Blot was applied to detect expression of MORF in IP-PDLSCs. Quantitative RT-PCR, Western Blot and alizarin red staining were applied to determine osteogenie differentiation potential of H-PDLSCs with MORF knock- down. Results： Quantitative RT-PCR and Western Blot showed lower expression of MORF in P-PDLSCs compared with H-PDLSCs （P 〈0.05）. Western Blot revealed lower expression of MORF in IP-PDLSCs. Quantitative RT-PCR, Western Blot and alizarin red stai- ning indicated osteogenie differentiation potential was inhibited in H-PDLSCs with MORF knockdown（ P 〈 0.05 ）. Conclusion： Peri- odontitis can suppress the expression of MORF in PDLSCs and inhibite the osteogenic differentiation potential of PDLSCs.