中文摘要：

目的：通过比较正常与炎症来源牙周膜干细胞（PDLSCs）中乙酰基转移酶KAT2A表达水平的差异,研究乙酰基转移酶KAT2A在PDLSCs中对成骨分化的调控。方法：有限稀释法克隆化培养出正常与炎症来源的PDLSCs;基因与蛋白检测方法对比两种来源PDLSCs中KAT2A的表达水平;基因、蛋白检测与茜素红染色方法对比正常PDLSCs及干扰KAT2A基因后的PDLSCs成骨表达的差异。结果：与健康来源PDLSCs相比,炎症来源PDLSCs中KAT2A的表达显著下降,差异有统计学意义（P〈0.05）;KAT2A基因被干扰后,PDLSCs成骨能力下降,差异有统计学意义（P〈0.05）。结论：牙周炎会导致PDLSCs中KAT2A表达的下降,导致细胞成骨分化受到抑制。

英文摘要：

Objective：To compare the ievei of acetyitransferase KAT 2A in periodontai iigament stem ceiis derived from heaithy indi-viduais （ H-PDLSCs） with periodontai iigament stem ceiis derived from periodontitis individuai （ P-PDLSCs ） , and to determine the effect of acetyitransferase KAT 2A on osteogenic differentiation potentiai of PDLSCs .Methods：Human PDLSCs were cuitured and cioned with iimited diiuted method .Quantitative RT-PCR and Western Biot were used to examine different expression of acetyitransferase KAT2A in two hinds of PDLSCs.Quantitative RT-PCR,Western Biot and Aiizarin Red Staining were used to determine osteogenic dif-ferentiation potentiai of PDLSCs with gene KAT 2A hnochdown.Results：Quantitative RT-PCR and Western Biot showed that iower ex-pression of KAT2A was detected in P-PDLSCs compared with that of H-PDLSCs,with statisticai significance（P〈0.05）.Quantitative RT-PCR,Western Biot and Aiizarin Red Staining indicated that osteogenic differentiation potentiai was inhibited in H -PDLSCs with KAT2A hnochdown and the effect was statisticaiiy significant （ P〈0.05）.Conclusions：Periodontitis might repress expression of KAT2A in PDLSCs,which associates with osteogenic differentiation potentiai .