中文摘要：

[目的]探讨氟、砷染毒对大鼠雄性仔鼠海马组织中Ⅰ组代谢型谷氨酸受体m Glu R1α和m Glu R5蛋白表达的影响。[方法]无特定病原体（SPF）SD大鼠80只,雌雄按1∶1交配,将观察到有阴栓形成的孕鼠随机分为对照组、氟染毒组、砷染毒组及氟砷联合染毒组,每组10只,从受孕第0天开始分别饮用自来水、100 mg/L NaF、75 mg/L NaAsO_2及100 mg/L NaF＋75 mg/L NaAsO_2的混合水溶液,连续染毒至子代大鼠断乳（出生后21天,PND21）,断乳后仔鼠饮用与母鼠一样的染毒溶液至出生后42天（PND42）。采用高效液相色谱法检测仔鼠海马组织中谷氨酸（Glu）与γ-氨基丁酸（GABA）的含量,Western blot法检测海马组织中m Glu R1α和m Glu R5蛋白表达水平。[结果]与对照组相比,砷染毒组及氟砷联合染毒组PND21和PND42仔鼠海马组织中Glu及PND42仔鼠GABA含量明显下降（P〈0.05）。与氟染毒组比较,PND42氟砷联合染毒组仔鼠海马组织中Glu含量明显降低（P〈0.05）。砷染毒组及氟砷联合染毒组PND21仔鼠,氟、砷染毒组及氟砷联合染毒组PND42仔鼠海马组织m Glu R1α蛋白表达低于对照组（P〈0.05）;氟、砷染毒组及氟砷联合染毒组PND21仔鼠,砷染毒组及氟砷联合染毒组PND42仔鼠海马组织mGluR5蛋白表达低于对照组（P〈0.05）;与氟染毒组相比,氟砷联合染毒组PND21和PND42仔鼠m Glu R1α蛋白表达均降低（P〈0.05）。[结论]生命早期氟、砷染毒及氟砷联合染毒均可下调雄性子代大鼠海马组织中m Glu R1α和m Glu R5蛋白表达,氟砷联合染毒下调的最明显。

英文摘要：

[ Objective ] To measure the protein expressions of group I metabotropic glutamate receptors （mGluRs）, including mGluRla and mGluR5 in hippocampus after exposure to fluoride, arsenic, and combination in male offspring rats. [ Methods ] Specific pathogen free SD rats （n=80） were mated with a 1 ： 1 male-to-female ratio. The pregnant rats were randomly assigned to four groups： control, arsenic, fluoride, and combination groups, with 10 rats each. The control rats drank tap water. Rats in the three exposure groups drank water with sodium fluoride （100 mg/L）, sodium arsenite （75 mg/L）, and sodium fluoride （100 mg/L） plus sodium arsenite （75 mg/L） combination from day 0 of pregnancy to postnatal day 21 （PND21） of pups. The weaned pups drank the same water as mother rats till postnatal day 42 （PND42）. The contents of glutamate （Glu） and gamma aminobutyric acid （GABA） in hippocampus of pups were detected by high performance liquid chromatography. The protein expressions of mGluRla and mGluR5 were detected by Western blot. [ Results ] Compared with the control group, the levels of Glu in hippocampus in the PND21 and PND42 pups and the level of GABA in the PND42 pups of the arsenic group and the combination group were decreased （P 〈 0.05）. Compared with the fluoride group, the level of Glu in hippocampus in the PND42 pups was decreased （P〈0.05）. The protein expression of mGluRla decreased in hippocampus in the PND21 pups in the arsenic group and the combination group as well as in the PNIM2 pups in the fluoride, arsenic and combination groups （P〈0.05）. The protein expression of mGluR5 decreased in hippocampus in the PND21 pups in the fluoride, arsenic, and combination groups and in the PND42 pups in the arsenic and combination groups （P〈 0.05）. The protein expression of mGluRla in the PND21 and PND42 pups in the combination group was lower than that in the fluoride group （P〈 0.05）. [ Conclusion ] Fluoride and arsenic exposure, either single or combin