中文摘要：

目的探讨生命早期氟、砷单独及联合暴露对大鼠子代空间学习记忆能力的影响。方法将48只新出生SPF级sD雄性大鼠随机分为对照（自来水）组、氟（100mg／L氟化钠）染毒组、砷（75mg／L亚砷酸钠）染毒组和联合（100mg／L氟化钠＋75mg／L亚砷酸钠）染毒组，每组12只。从受孕第0天至仔鼠生后21d（postnatal day 21，PND21），孕鼠采用自由饮水方式进行染毒；自PND22（断乳后）～PND42，仔鼠采用自由饮水方式继续染毒。采用Morris水迷宫实验测试PND21和PND42仔鼠的空间学习记忆能力。结果砷染毒组和联合染毒组PND21和PND42仔鼠逃避潜伏期（第4天）均显著长于对照组（P〈0．05），联合染毒组PND42仔鼠逃避潜伏期（第4天）显著长于氟和砷染毒组（P〈0．05）。与对照组相比，氟、砷及联合染毒组PND21和PND42仔鼠的首次达台时间明显延长，目标象限时间明显缩短（P〈0．05），PND42仔鼠穿台次数明显少于对照组（P〈0．05）。氟、砷染毒组以及联合染毒组PND21与PND42仔鼠的逃避潜伏期、首次达台时间、目标象限时间、穿台次数比较，差异均无统计学意义。结论生命早期氟、砷单独及联合暴露可损伤仔鼠空间学习记忆能力。

英文摘要：

Objective To know the effects of fluoride,arsenic individually and in combination exposure on spatial learning and memory in rat offspring during early life. Methods Forty-eight newborn specefic-pathogen-free SD male rats were randomly divided into control and experimental groups as follows： control group （drinking tap water）, fluoride group （drinking the fluoridated water of 100 mg/L sodium fluoride）, arsenic group （drinking the water containing 75 mg/L sodium arsenite）, fluoride plus arsenic group （100 mg/L sodium fluoride and 75 mg/L sodium arsenite solution）, and 12 pups in each group. The pregnant rats were treated with fluoride or arsenic through drinking water ad libitum from pregnant day 0 to postnatal day 21（PND21）. The pups were given the same treatment as their parental generation after PND22 until PND42. The ability of spatial learning and memory of pups were measured using Morris water maze test. Results The escape latency periods on training day 4 of PND21 and PND42 pups in arsenic and fluoride plus arsenic group were significantly higher than control pups （P〈0.05）. The escape latency period （training day 4） of PND42 pups in fluoride plus arsenic group was significantly higher than fluoride and arsenic alone group （P〈0.05）. Compared with the control pups,the first platform time of PND21 and PND42 pups exposed to fluoride and arsenic in single use and in combination significantly prolonged,the target quadrant time markedly shortened （P〈0.05）, and the frequency through the platform within 120 s of PND42 pups were all much less than control pups （P〈0.05）. The differences of escape latency,the first platform time, the target quadrant time and the frequency through the platform between PND21 and PND42 pups in the three exposure groups were not statistically significant. Conclusion Fluoride and arsenic exposure, either in single use or in combination may damage the ability of spatial learning and memory in rat offspring during early life. Key words： Flu