中文摘要：

目的探讨广东地区汉族人群Klotho G-395A（rs1207568）基因多态性与冠心病遗传易感的关系,为冠心病的防治提供新思路。方法在中国广东地区汉族人群中随机选择340例冠心病患者和280例健康对照个体,采用聚合酶链式反应-连接酶检测反应（PCR-LDR）分析技术,对Klotho G-395A多态位点进行分型,采用非条件逻辑回归分析统计该多态位点与冠心病遗传易感的相关性。结果 GG、AG、AA基因型在冠心病组中的分布频率分别为69.1%、29.1%和1.8%,在对照组中分别是73.2%、23.9%和2.9%,两组间基因型频率分布差异无统计学意义（2=2.719,P=0.257）。非条件逻辑回归分析发现,A变异等位基因并没有增加个体患冠心病的发病风险（OR=1.12,95%CI 0.82～1.53,P=0.466）。此外,对年龄、性别、饮酒和吸烟情况等进行分层分析显示,Klotho G-395A多态与冠心病的易感无相关性。结论 Klotho G-395A多态位点与广东地区汉族人群冠心病遗传易感无相关性。

英文摘要：

Objective To investigate the correlation between the Klotho G-395A（rs1207568） polymorphism and the risk of coronary artery disease（CAD） in ethnic Han Cantonese population.Methods The genotypes of the Klotho G-395A polymorphism were determined in 340 CAD patients and 280 controls by polymerase chain reaction-ligase detection reaction（PCR-LDR）.The correlaion between the polymorphism and the risk of CAD was evaluated using unconditional logistic regression analysis.Results The frequencies of GG,AG and AA genotypes in the case subjects were 69.1%,29.1% and 1.8%,respectively;and 73.2%,23.9% and 2.9%,respectively,in the control subjects.No significant difference was observed in the frequency of Klotho G-395A genotypes between cases and controls（χ2 = 2.719,P = 0.257）.No significantly increased risk of CAD was observed for those carrying A variant allele（OR = 1.12,95% CI 0.82 ～ 1.53,P = 0.466）.Moreover,there was no significant correlation between this polymorphism and CAD risk among subgroups by age,sex,drink,smoking status,etc.Conclusion Klotho G-395A polymorphism is not associated with CAD in ethnic Han Cantonese population.