中文摘要：

本文用浓硝酸活化多层碳纳米管，将壳聚糖与活化后的碳纳米管制备成复合材料，并将其滴涂于玻碳电极表面，制备出烟酰胺腺嘌呤二核苷酸（NADH）的电化学传感器。采用循环伏安法研究了该传感器的电化学性质以及对NADH的电催化氧化行为。实验结果表明，NADH在该电极上于＋0.37V（vs．SCE）左右出现一氧化峰，与未修饰的玻碳电极相比，该修饰电极明显降低了NADH的氧化峰电位，消除了反应中间产物对电极表面的污染问题。对实验条件进行了优化，建立了碳纳米管／壳聚糖修饰电极微分脉冲伏安法测定NADH的方法。本文方法具有较好的重现性和选择性，对NADH检测的线性范围为1.0×10^-4～9.0×10^-3mol／L，检测限为9．2×10^-5mol／L。

英文摘要：

Muff-wall carbon nanotubes were activated in concentrated nitric acid. Chitosan and ＇activated eabon- nanotube were prepared into composite material. A glassy carbon electrode was modified by pipetting a aliquot solution of the material onto the electrode surface to prepare electroeatalytie sensor of β - nieotinamide adenine dinueleotide （NADH）. The electrochemical properties of the sensor and its eleetrocatalytie oxidation behaviours towards NADH.were studied by cyclic voltammetry. Experimental results indicated that NADH produced an electrochemical oxidation peak at about ＋0. 37V（vs. SCE）. In-comparison with a bared glassy carbon electrode, the oxidation peak potential of NADH at the modified electrodes proposed by this paper was substantially reduced. The problem of electrode fouling caused by reaction intermediate was eliminated. The experiment conditions were optimized and the determination method of NADH by differential pulse voltammetry using carbon - nanotubes/ehitosan modified electrodes was established. The method proposed by this paper possess good reproducibility and selectivity. A linear response to NADH in the concentration range of 1.0 × 10^-4～9. 0× 10^-3 mol/L was observed, with a detection limit of 9. 2× 10^-5 mol/L