中文摘要：

根据GenBank注册的AJ272108等序列，利用Oligo6应用软件设计内外2对引物，采用RT—PCR技术对本实验室分离的猪戊型肝炎病毒进行探索性扩增，并对测定的序列进行分析，结果用套式引物扩增出了目的条带，序列分析表明该序列属于基因Ⅳ型，并与其核苷酸同源性在85．6％～94．8％之间，氨基酸同源性在99．1％～100％之间。而与基因Ⅲ型核苷酸同源性最低，与基因Ⅱ型氨基酸同源性最低。

英文摘要：

According to GenBank registered AJ272108 sequence, etc, and referring the literatures of Meng and other reSearchers,then using Oligo6 software to design two pairs of primers, using RT-PCR technology to amplificate swine hepatitis E virus which was isolated from the laboratory and analysising the sequence. The results showed that using Nested primers to expand the fragment,and sequence analysis showed that the gene sequence belong to genotypes 4,and with genotypes 4 between 85.6% and 94.8% in nucleotide identity,between 99.1% and 100% in the amino acid identity. But keeping the minimum with genotypes 3 in nucleotide identity,and keeping the minimum with genotypes 2 in amino acid identity.