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中文摘要：
参照GenBank公布的鹅源新城疫病毒NA-1株F基因（DQ659677）的核苷酸序列设计1对引物，通过PCR扩增F基因全长，将其克隆到pET-22b（＋）载体中，构建了原核表达载体pET-22b（＋）-F，转化大肠杆菌BL21（DE3），表达并纯化了重组蛋白，Western-blot证明该重组蛋白具有免疫原性。利用重组蛋白为包被抗原，通过方阵试验确定了包被抗原的最佳包被量为0．21ug／孔，建立了检测鹅源新城疫病毒抗体的间接ELISA诊断方法。
英文摘要：
According to the F gene sequences of Newcastle disease virus from goose （DQ659677） available in GenBank,a pair of primers was designed for F gene of NDV from goose. Then the amplified product was cloned into prokaryotic expression vector pET-22b（＋） and over-expressed in E. coli BL21 （DE3）. Western-blot analysis showed that the purified recombinant protein had good immunological activity. The optimal concentration of coated antigen F recombinant protein was 0.21ug per well,which was determined by phalanx titrimetry. Then an indirect ELISA was established for detection of the antibody against Newcastle disease virus from goose.
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