中文摘要：

目的：通过体内动物实验研究CD59-siRNA对卵巢癌移植瘤CD59的沉默效应及其抑瘤作用,探讨CD59在肿瘤免疫逃逸中的作用。方法：采用脂质体转染法将CD59干扰质粒（T组）和空质粒（V组）转染A2780细胞,获得稳定表达细胞株,将前两组A2780细胞和未转染（C组）的A2780细胞分别接种于裸鼠皮下建立肿瘤模型,通过绘制肿瘤生长曲线、RT-PCR和Western Blot研究其抑瘤效应及对CD59的沉默效应。结果：肿瘤生长曲线显示,与对照组相比,CD59干扰质粒转染组肿瘤生长明显受抑制（P〈0.05）。RT-PCR和Western Blot结果表明,干扰组的CD59mRNA及CD59蛋白与对照组相比显著降低（P〈0.05）。结论：动物实验表明,特异性沉默CD59基因的siRNA表达载体可以明显抑制CD59的表达及卵巢癌在体内的生长,进一步说明了CD59在肿瘤免疫逃逸中的作用。

英文摘要：

Objective：To investigate the inhibition effect of CD59-siRNA in vivo on CD59 gene and the growth of ovarian cancer,and to investigate the role of CD59 in tumor immune escape.Methods： A2780 cells transfected with shRNA plasmid（T group）,A2780 cells transfected with blank plasmid（V group） and A2780 cells（C group） were subcutaneously injected into nude mice respectively.The inhibition effect of CD59-siRNA on CD59 and tumor was evaluated by tumor growth RT-PCR and Western Blot.Results： That the growth of cells transfected with shRNA plasmid was significantly inhibited （P〈0.05）. RT- PCR and Western Blot showed that the expression of CD59 mRNA and CD59 protein in T group decreased significantly compared with other groups （P〈0.05）. Conclusion： CD59-siRNA significantly inhibited the tumor growth and the expression of CD59. The results may further indicate the role of CD59 in tumor immune escape.