中文摘要：

目的：观察海马及杏仁核α-氨基羟甲基恶唑丙酸（AMPA）受体亚基和相关调节蛋白在束缚应激状态下蛋白表达变化及逍遥散的调节作用。方法：使用每天捆绑3 h的方法制作慢性束缚应激动物模型,并用逍遥散进行干预,分别于7 d后和21 d后用Western blot方法检测各组大鼠海马CA1区、CA3区、齿状回（DG）和杏仁核的AMPA受体亚基GluR2/3及N-乙基顺丁烯二酰亚胺敏感性的融合蛋白（NSF）、PKC作用蛋白1（PICK1）蛋白表达的情况。结果：7 d应激可使DG和杏仁核的GluR2/3、NSF表达显著降低（P均〈0.05）,使PICK1在CA1区的表达量显著增多（P〈0.05）,逍遥散对PICK1变化显示出一定调节作用。21 d应激可使CA1区的GluR2/3、NSF表达升高,其中GluR2/3有显著性差异（P〈0.01）,而在杏仁核表达有降低趋势,逍遥散对其均有显著调节作用（均为P〈0.05）,21 d应激使杏仁核PICK1表达量出现升高趋势,逍遥散可显著降低其表达（P〈0.05）。结论：AMPA受体在短期重复应激和慢性应激状态下反应不同,海马和杏仁核反应相反,逍遥散对慢性应激状态下AMPA受体表达的调节作用较短期重复应激强。

英文摘要：

Aim： To observe protein expression changes of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid（AMPA） receptor and related regulatory protein in the hippocampus and amygdala in chronic immobilization stressed rat and Xiaoyaosan＇s regulatory effect. Methods： Rats were tied 3 h per day to establish immobilization stress condition and treatment with Xiaoyaosan. After 7 days and 21 days stress, the protein expression of AMPA receptor subunit（GluR2/3）, N-ethylmaleimide sensitive factor（NSF） and protein interacting with C-kinase 1 （PICK1） in hippocampus and amygdala were detected by using Western blot techniques. Results： The expression of GluR2/3,NSF in dentate gyrus（DG） and amygdala were markedly attenuated（P ：b：J 〈 0.05） and PICK1 in CA1 region were significantly increased（ P 〈 0.05） in 7 d immobilization stressed rats while 7 days xiaoyaosan treatment showed an effective regulatory result to PICK1 ＇s changes. Under 21 days immobilization stressed condition, the expression of GluR2/3, NSF in CA1 region showed an increasing trend, and GluR2/3 showed a markedly increase（ P 〈 0.01 ）, but showed an significantly decreased trend in amygdala, Xiaoyaosan showed an effective result to such changes above（ P 〈 0.05 ）. The expression of PICK1 showed increasing trend in amygdala and xiaoyaosan could lower its expression（ P 〈 0.05）. Conclusion： There are different trends of the expression of AMPA receptor in repeat short-term stress versus chronic immobilization stress, and in hippocampal CA1 region versus amygdala. Xiaoyaosan has better regulation effect on the expression of AMPA receptors in the condition of chronic immobilization stress than those of repeat shortterm stress.