中文摘要：

大气CO2浓度升高可能对森林土壤的甲烷（CH4）氧化速率产生影响.本文采用开顶箱技术,对连续6年高浓度CO2（500μmol·mol-1）处理的长白山森林典型树种蒙古栎树下土壤CH4氧化速率进行研究,并利用CH4氧化菌的16SrRNA特异性引物以及CH4单加氧酶功能基因引物分析了土壤中CH4氧化菌的群落结构与数量.结果表明：CO2浓度增高后,生长季土壤甲烷氧化量与对照和裸地相比分别降低了4%和22%;基于16SrRNA特异性引物的DGGE分析表明,CO2浓度增高导致两类甲烷氧化菌的多样性指数降低;CO2浓度增高对土壤中Ⅰ类甲烷氧化菌数量无显著影响,而使土壤中Ⅱ类甲烷氧化菌数量显著减少,功能基因pmoA拷贝数与对照和裸地相比分别降低了15%和46%.CO2浓度增高导致森林土壤甲烷氧化菌数量与活性降低,土壤含水量的增加可能是导致这一现象的主要原因.

英文摘要：

Elevated atmospheric CO2 concentration may affect the oxidation rate of methane （CH4） in forest soil.In this study,the effects of a 6-year exposure to elevated CO2 concentration （500 μmol·mol-1） on the soil microbial process of CH4 oxidation under Quercus mongolica seedlings were investigated with open top chamber （OTC）,and specific 16S rRNA and pmoA gene fragment primers were adopted to analyze the diversity and abundance of soil methanotrophs.Comparing with that under ambient CO2 and open-air,the soil methane consumption under elevated atmospheric CO2 during growth season was reduced by 4% and 22%,respectively.The specific 16S rRNA PCR-DGGE analysis showed that under elevated CO2,the community structure of methane-oxidizing bacteria （MOB） changed,and the diversity index decreased.Elevated CO2 concentration had no distinct effects on the abundance of TypeⅠ MOB,but decreased the amount of TypeⅡ MOB significantly.The pmoA gene copy number under elevated CO2 concentration decreased by 15% and 46%,respectively,as compared with that under ambient CO2 and open-air.Our results suggested that elevated atmospheric CO2 decreased the abundance and activity of soil methanotrophs,and the main cause could be the increase of soil moisture content.