中文摘要：

【目的】明确草莓炭疽菌（Colletotrichum fragariae）在侵染草莓叶片过程中病原菌的侵染致病过程,为防控草莓炭疽病提供理论依据。【方法】用草莓炭疽菌绿色荧光蛋白标记菌株LC0220-7GFP的分生孢子悬浮液接种离体健康草莓叶片,在荧光显微镜下观察病原菌的侵染过程及侵染结构。【结果】接种6~9 h为病原菌分生孢子萌发高峰期,接种9 h约90.00%的分生孢子已萌发;接种12~24 h为侵染结构形成高峰期,接种24 h约70.00%的芽管顶端产生附着胞并形成侵染钉开始侵染草莓叶片表皮细胞,有少量的菌丝开始直接侵染叶片表皮细胞,同时在寄主上表皮上有少量的附着枝形成;接种48 h为菌丝形成高峰期,菌丝大量形成并沿着表皮细胞延伸成网络状,叶片开始出现零星病斑;接种72~96 h为分生孢子盘形成高峰期;接种96~120 h为产孢高峰期,接种96 h产生新的分生孢子,大部分病原菌完成一个侵染循环;接种144 h形成典型的炭疽病斑。【结论】草莓炭疽菌通过产生附着胞侵入或直接侵入草莓叶片表皮细胞,使草莓叶片发病,形成典型的炭疽病斑。

英文摘要：

[Objective]The present experiment was conducted to the infection process of strawberry by Colletotrichum fragariae, in order to provide scientific basis for controlling strawberry anthraenose. [Method]The healthy strawberry leaves were inoculated with conidial suspension of C. fragariae green-fluorescent-protein-marked strain LC220-7GFP. The infection process and pathogenicity were observed by fluorescence microscope. [Result]The results showed that, the crest-time of eonidial germination was within 6-9 h after inoculation, with 90.00% eonidia germinated at 9 h after inoculation. The crest-time of formation of infection structures was within 12-24 h after inoculation, and about 70.00% germ tubes generated classical appressoria, and appressoria formed infection pegs to penetrate epidermal cells of leaves, only a spot of hyphae directly infected epidermal cells of leaves, meanwhile a little hyphopodium was formed on epidermal cells at 24 h. The crest-time of hyphae formation was at 48 h after inoculation, the hyphae had extensively colonized and formed a network on the leave surfaces, and sporadic disease spots started to appear. The crest-time of acervulus and conidia formation was within 72-96 h and 96-120 h after inoculation, respectively, and most of pathogenic bacteria completed the disease cycle at 96 h. Typical anthracnose disease spots could be found on the leaves at 144 h after inoculation. [Conclusion]C. fragariae can cause anthracnose of strawberry leaves by directly invading epidermal cells or first forming appressoria then invading epidermal cells, and typical disease spots can be found on the leaves.