中文摘要：

运用双向凝胶电泳技术、MALDI-TOF-TOF/MS质谱鉴定和数据库检索,分析发菜锰过氧化氢酶（Mn-CAT）在干燥和复吸水后的差异表达水平,根据Mn-CAT鉴定的已知氨基酸序列设计简并引物克隆该基因,并研究其原核表达.结果表明：干燥发菜复吸水后Mn-CAT表达量明显高于干燥状态下的表达量.使用简并引物克隆获得长度为693 bp的Mn-CAT基因（GenBank登录号为GU549477）.将Mn-CAT基因在大肠杆菌中表达,获得1个约26 kD的外源蛋白,经Western blotting验证,该外源蛋白为Mn-CAT.研究结果为进一步研究发菜耐旱的分子机理、探讨发菜对极端干旱环境的适应机制奠定了基础.

英文摘要：

2-DE and MALDI-TOF-TOF/MS were performed to compare and identify the Mn-CAT expression level from Nostoc flagelliforme subjected to desiccation and rehydration.Degeneracy primers were designed based on identified amino acid sequences in order to clone Mn-CAT gene,and prokaryotic expression of Mn-CAT gene was carried out.The results indicated that Mn-CAT expression level of N.flagelliforme subjected to rehydration was higher than that to desiccation.Mn-CAT gene was cloned and a full length of 693 bp DNA was obtained（GenBank accession number GU549477）.Mn-CAT gene was expressed in E.coli,and a 26 kD heterologous protein was observed.Western blotting confirmed that the protein was Mn-CAT.The results laid a foundation of drought-resistant molecular mechanism and protection mechanism of N.flagelliforme in extreme arid environment further more.