中文摘要：

这研究的目的是为产生猪的 tetraploid (4n ) 优化 electrofusion 条件胚胎并且生产 tetraploid/diploid (4n/2n ) 妄想的胚胎。不同电场紧张被测试， 2 直接电流(DC ) 为 30 0.9 kV/cm 搏动 ? s 作为最佳条件被选择让 2 房间胚胎的 electrofusion 生产 4n 胚胎。2 房间胚胎的熔化率和到胚囊的发展率大概 4n 胚胎，分别地到达了 85.4% 和 28.5% 。68.18% 熔化胚胎被发现是示威了由的 4n 在 situ 杂交(鱼) 荧光灯。尽管在 4n 胚囊的分裂球的数字比在 2n 胚囊显著地低( P < 0.05 )，处于在 2n 和 4n 胚胎之间的胚囊的发展的率没有重要差别( P > 0.05 )，建议在 4n 胚胎形成能力的胚囊类似于在 2n 胚胎的那些。而且， 4n/2n 妄想的胚胎被 4n 和 2n 胚胎的聚集获得。我们发现发展的率和房间4房间( 4n )的胚囊数妄想的胚胎是的 /4-cell ( 2n )比那些显著地高2房间( 4n ) /4-cell ( 2n )，4房间( 4n ) /8-cell ( 2n )，4房间( 4n ) /2-cell ( 2n )妄想的胚胎( P < 0.05 )。与老鼠怪物一致， 4n 房间的多数贡献 trophectoderm (TE ) ，当 2n 房间主要是在内部房间的现在时，集中(ICM ) 猪的 4n/2n 妄想的胚胎。我们的学习建立了一条可行、有效的途径生产猪的 4n 胚胎和 4n/2n 妄想的胚胎。

英文摘要：

The aim of this study was to optimize electrofusion conditions for generating porcine tet- raploid （4n） embryos and produce tetraploid/diploid （4n/2n） chimeric embryos. Different electric field intensities were tested and 2 direct current （DC） pulses of 0.9 kV/em for 30 p.s was selected as the optimum condition for electrofusion of 2-cell embryos to produce 4n embryos. The fusion rate of 2-cell embryos and the development rate to blastocyst of presumably 4n embryos, reached 85.4% and 28.5%, respectively. 68.18% of the fused embryos were found to be 4n as demonstrated by fluorescent in situ hybridization （FISH）. Although the number of blastomeres in 4n blastocysts was significantly lower than in 2n blastocysts （P 〈 0.05）, there was no significant difference in developmental rates of blastocysts between 2n and 4n embryos （P 〉 0.05）, suggesting that the blas- tocyst forming capacity in 4n embryos is similar to those in 2n embryos. Moreover, 4n/2n chimeric embryos were obtained by aggregation of 4n and 2n embryos. We found that the developmental rate and cell number of blastocysts of 4-cell （4n）/4-cell （2n） chimeric embryos were significantly higher than those of 2-cell （4n）/4-cell （2n）, 4-cell （4n）/8-cell （2n）, 4-cell （4n）/2-cell （2n） chimeric embryos （P 〈 0.05）. Consistent with mouse chimeras, the majority of 4n cells contribute to the trophectoderm （TE）, while the 2n cells are mainly present in the inner cell mass （ICM） of porcine 4n/2n chimeric embryos. Our study established a feasible and efficient approach to produce porcine 4n embryos and 4n/2n chimeric embryos.