中文摘要：

胚胎生殖细胞（EGCs）是原始生殖细胞（PGCs）在体外特定条件下,经重编程而形成的、具有和胚胎干细胞（ESCs）相似特性的多能性干细胞。猪EGCs的研究在利用EGCs进行核移植、生产转基因动物以及建立生物反应器方面具有广阔的应用前景。然而到目前为止真正意义上的猪EG细胞系还没有建立,体外培养条件不确定是影响猪EGCs建系的重要因素之一。本研究探讨了猪PGCs体外培养过程中不同信号通路抑制剂以及不同饲养层对其重编程形成EGCs的影响,确定了CHIR99021、SB431542和小鼠成纤维细胞制作的饲养层组合,能够较大程度维持PGCs的增殖及存活能力,实现向EGCs的重编程。

英文摘要：

Embryonic germ cells（EGCs） which have similar properties to embryo stem cells（ESCs） are pluripotent stem cells reprogrammed from primordial germ cells（PGCs） under certain conditions in vitro. The study on pig EGCs has great value and promising prospects in nuclear transfer, producing genetically modified animals and establishing bioreactor. However, the truly pig EG cell line has not been established yet, one of the most important influence factors is the culture condition in vitro. In this study, we investigated the effect of different cell signaling pathway inhibitors and feeder layers on the pig EGCs and confirmed that the combination of CHIR99021, SB431542 and MEF feeder layer was optimized condition for PGCs proliferation, survival and reprogramming ability to EGCs.