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中文摘要：
采用Ficoll密度梯度离心法得到人外周血单个核细胞（PBMC）,并结合磁珠分选的方法进一步纯化得到正常B淋巴细胞,探索了正常和肿瘤B淋巴细胞之间的差异。通过应用具有高分辨率的原子力显微镜（AFM）对正常人和慢性淋巴白血病人外周血B淋巴细胞进行成像,并对这两种B淋巴细胞的高度、直径、体积及膜表面的颗粒平均高度、平均粗糙度和颗粒分布进行测量,对比观察两组细胞膜表面宏观和纳米结构的变化。结果表明,慢性淋巴白血病B淋巴细胞比正常的B淋巴细胞高大,细胞膜表面颗粒更大且细胞膜粗糙。此外,对这两组淋巴细胞进行了机械性质方面的测量和统计,结果发现慢性淋巴白血病B淋巴细胞粘附力（524.1±160.0）pN比正常B淋巴细胞粘附力（1091±260）pN约小1倍,且癌变的B淋巴细胞硬度明显比正常的小。当正常细胞癌变时,细胞的形貌、超微结构及骨架会发生一定的改变。实验证明应用AFM可在形态学和机械性质上明显区别正常和慢性淋巴白血病B淋巴细胞,为临床诊断慢性淋巴白血病提供新的技术手段。
英文摘要：
Normal peripheral blood B lymphocyte（BL）was obtained and purified by the Ficoll isolation method and the magnetic cell sorting method,respectively.Based on the fact that the morphology and ultrastructure of the cancerated cells were different from those of the normal ones,a high resolution atomic force microscope（AFM）was used to detect the differences between the human normal peripheral blood BL and the chronic lymphocytic leukemia B lymphocyte（CLLBL）in terms of height,diameter and volume of lymphocyte and the average height and roughness of the particles on the membrane surface,and the particle distribution on the cell membrane surface.The data showed that the CLLBL were bigger,the particles on the cell membrane surface became larger and the cell membrane was rougher in comparison with those of normal BL.The mechanical properties of these two groups of BL were also measured and the data showed that the adhesion force of CLLBL was nearly one half of that of the normal BL,and the hardness of CLLBL was lower than that of normal BL.The experimental results have shown that AFM was a useful means for clinically diagnosis of CLBL.
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