中文摘要：

从内蒙古传统干酪中分离到的1株产细菌素屎肠球菌（Enterococcus faecium）M-2为试材,对其所产细菌素进行提取、纯化和理化特性分析。经80%硫酸铵盐析及SPSepharose Fast Flow阳离子交换树脂层析得到纯化的细菌素,其比活力可达411.204AU/mg;Tricine SDS-PAGE电泳结果显示其分子量约为3400D;特性分析发现该细菌素有很好耐热和酸碱性,经121℃处理20min后仍有活性残留,且在pH2-10的范围内活性稳定;吐温20和吐温80对其活性无影响,而SDS、司本80和尿素可导致活性损失40%-50%;蛋白酶K和胰蛋白酶处理可使其完全失活,而木瓜蛋白酶和中性蛋白酶仅可导致部分失活;871AU/mL肠球菌素M-2对单增李斯特氏菌（Listeria monocytogenes）的作用方式为杀菌;该细菌素抑菌谱较广,不仅对李斯特氏菌（Listeria spp.）,葡萄球菌（Staphylococcus spp.）,芽胞杆菌（Bacillus spp.）等革兰氏阳性菌有明显抑制作用,同时对大肠杆菌（Escherichiacoli）及假单胞菌（Pseudomonas spp.）.等阴性菌也表现出抑菌活性，有作为食品防腐剂的良好应用前景。

英文摘要：

Purification and characterization of an antilisteria bacteriocin produced by Enterococcus faecium M-2, isolated from Inner Mongolia traditional cheese, were studied.Enterocin M-2 was purified by 80% ammonium sulphate precipitation, followed by SP-Sepharose FF cation exchange chromatography.And the specific activity of purified bacteriocin was 411.204 AU/mg.The inhibitory activity was heat-stable and still remained after 20 min at 121 ℃.The activity remained stable at pH values from 2 to 10.Tween 20 and tween 80 did not decrease the activity.However, SDS, span 80 and urea induced 40%～50% activity loss.Enterocin M-2 could be inactivated by proteinase K and trypsin, and partially inactivated by papain and neutral proteinase.Mode of action of enterocin M-2 against Listeria monocytogenes is bactericidal at 871 AU/mL.In addition, the bacteriocin had a broad inhibitory spectrum.It not only had a strong effect on gram-positive, such as Listeria spp., Staphylococcus spp., Bacillus spp.and etc, but also could inhibited several gram-negative bacteria, such as Escherichia coli, Pseudomonas spp.and etc.These results show that the bacteriocin has a potential application as food additives and bio-preservatives in food industry.